96 Tests PN: B511113

Components:

45x Ab-conjugated beads (S5P8 - Canine IL-2 ab-bead). PN: B511113A. One vial containing 100 μL of anti-canine IL-2 conjugated to AimPlex Bead S5P8.

25x Biotin-detection Ab (canine IL-2 Biotin-dAb). PN: B511113B. One vial containing 100 μL of biotinylated anti-canine IL-2.

Lyophilized Standard Mix-Canine Group 1 Panel A, 10-Plex. PN: KG10010. One vial containing lyophilized recombinant IFNγ, IL-2, IL-6, IL-8, IL-10, IL-12p40, IL-17A, MCP-1, SCF and TNFα. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

Application: Optimal antibody pair and antigen standard for assaying Canine IL-2.  Can be multiplexed with other analytes in Canine Group 1.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only. Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 5 pg/mL

  • Quantitation range:

  • LLOQ: < 10 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Canine Group 1: Negligible

  • Sample volume: 15 µL/test

Description:

Interleukin 2 (Accession Q29416) is a member of the interleukin cytokine family secreted primarily by activated CD4+ and CD8+ T-cells, NK cells, and dendritic cells. IL-2 participates in immune responses but also in delivering signals for thymic development, which in turn promotes homeostasis of T-cells. IL-2 promotes T-cell differentiation into effector, regulatory, or memory T-cells. Elevated levels of IL-2 may be correlated with blood hypereosinophilia, hemophagocytic lymphohistiocytosis, and a range of cardiopulmonary toxicities such as tachycardia and dyspnea.

References:

  1. Hayden A, Lin M, Park S, Pudek M, Schneider M, Jordan MB, Mattman A, Chen LYC. Soluble interleukn-2 receptor is a sensitive diagnostic test in adult HLH. Blood Advances. 2017; 1(26): 2529-2534. Doi: 10.1182/bloodadvances.2017012310

  2. Letourneau S, van Leeuwen EMM, Krieg C, Martin C, Pantaleo G, Sprent J, Surh CD, Boyman O. Il-2/anti-IL-2 antibody complexes show strong biological activity by avoiding interaction with IL-2 receptor α subunit CD25. PNAS. 2010; 107(5): 2171-2176. Doi:10.1073/pnas.0909384107.

  3. Liao W, Lin JX. Leonard WJ. IL-2 family cytokines: new insights into the complex roles of IL-2 as a broad regulator of T helper cell differentiation. Curr. Opin. Immunology. 2011; 23 (5): 598-604. Doi:10.1016/j.coi.2011.08.003.

  4. Malek TR, Castro I. Interleukin-2 Receptor Signaling: At the Interface between Tolerance and Immunity. Immunity. 2010; 33 (2): 153-165. Doi: 10.1016/j.immuni.2010.08.004.

  5. Prin L, Plumas J, Gruart V, Loiseau S, Aldebert D, Ameisen JC, Vermersch A, Fenaux P, Bletry O, Capron M. Elevated Serum Levels of Soluble Interleukin-2 Receptor: A Marker of Disease Activity in the Hypereosinophilic Syndrome. Blood Journal. 1991; 78: 2626-2632. ISSN: 1528-0020.