96 Tests PN: B113357

Components:

45x Ab-conjugated beads (S4P12- human sALCAM Ab-bead).PN: B11 3357A. One vial containing 100 µL of anti-human sALCAM conjugated to AimPlex Bead S4P12.

25x Biotin-detection (human sALCAM Biotin-dAb). PN: B113357B. One vial containing 100 µL of biotinylated anti-human sALCAM.

Lyophilized Standard Mix-Human Group B, 9-Plex. PN: HG9009B. One vial containing lyophilized recombinant human sCEACAM-1, sALCAM, sSLAMF4, sHVEM, sB7-DC, sB7-H1, sEpCAM, sBLAME and sEPOR.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

Application: Optimal antibody pair and antigen standard for assaying human Human ALCAM/sCD166. Can be multiplexed with other analytes in Human Group 9.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 10 pg/mL

  • Quantitation range:

  • LLOQ: < 20 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Human Group 9: Negligible

  • Sample volume: 15 µL/test

Description:

ALCAM is an antigen of the type I transmembrane glycoprotein, a member of the immunoglobulin superfamily of proteins. It is also called CD166, MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. Some literature sources have also cited it as the CD6 ligand (CD6L). It is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. CD166 protein expression is reported to be upregulated in a cell line deriving from a metastasizing melanoma. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intra-thymic T cell development. Recently, CD166 has also been used as a potential cancer stem cell marker.

References:

  1. Bowen MA, Patel DD, Li X, Modrell B, Malacko AR, Wang WC, Marquardt H, Neubauer M, Pesando JM, Francke U; et al. (Jun 1995). "Cloning, mapping, and characterization of activated leukocyte-cell adhesion molecule (ALCAM), a CD6 ligand". J Exp Med 181 (6): 2213–20. doi:10.1084/jem.181.6.2213. PMC 2192054. PMID 7760007.

  2. Joo, Y.-S., Singer, N. G., Endres, J. L., Sarkar, S., Kinne, R. W., Marks, R. M., Fox, D. A. Evidence for the expression of a second CD6 ligand by synovial fibroblasts. Arthritis Rheum. 43: 329-335, 2000. [PubMed: 10693872,]

  3. Saifullah, M. K., Fox, D. A., Sarkar, S., Abidi, S. M. A., Endres, J., Piktel, J., Haqqi, T. M., Singer, N. G. Expression and characterization of a novel CD6 ligand in cells derived from joint and epithelial tissues. J. Immun. 173: 6125-6133, 2004. [PubMed: 15528349,]