96 Tests PN: B117644

Components:

45x Ab-conjugated beads (S5P7 - human ANGPT-2/Angiopoietin-2/Tie2 ligand/ANG-2-bead). PN: B117644A. One vial containing 100 µL of anti-human ANGPT-2/Angiopoietin-2/Tie2 ligand/ANG-2 conjugated to AimPlex Bead S5P7.

25x Biotin-detection Ab (human ANGPT-2/Angiopoietin-2/Tie2 ligand/ANG-2 Biotin-dAb). PN: B117644B. One vial containing 100 µL of biotinylated anti-human ANGPT-2/Angiopoietin-2/Tie2 ligand/ANG-2.

Lyophilized Standard - Human ANGPT-2/Angiopoietin-2/Tie2 ligand/ANG-2. PN: B117644S. One vial containing lyophilized ANGPT-2/Angiopoietin-2/Tie2 ligand/ANG-2.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

Application: Optimal antibody pair and antigen standard for assaying human ANGPT-2/Angiopoietin-2/Tie2 ligand/ANG-2. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 20 pg/mL

  • Quantitation range:

  • LLOQ: < 50 pg/mL

  • ULOQ: > 20,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Sample volume: 15 µL/test

Description:

ANGPT-2/Angiopoietin-2/Tie2 ligand/ANG-2 is a growth factor belonging to the angiopoietin/Tie (tyrosine kinase with Ig and EGF homology domains) signaling pathway, one of the main pathways involved in angiogenesis.  ANG-2 was found to be an antagonist of Tie2 and an inhibitor of ANG-1. ANG-2 plays a central role in diseases related to vascular permeability and angiogenesis.  ANG-2 has also been shown to participate in several cancer-independent inflammatory conditions, such as autoimmune diseases, sepsis, and acute lung injury. Overexpression of ANG-2 has been found to correlate with increased angiogenesis, malignancy, and aggressive cancer growth.

References:

1.    Thurston G, et al. The complex role of angiopoietin-2 in the angiopoietin-tie signaling pathway. Cold Spring Harb. Perspect. Med. 2012;2: a006550.

2.    Scholz A, et al. Angiopoietin-2: A multifaceted cytokine that functions in both angiogenesis and inflammation. Ann. N. Y. Acad. Sci. 2015;1347:45–51.

3.    Akwii RG, et al.  Role of Angiopoietin-2 in Vascular Physiology and Pathophysiology.  Cells. 2019 May; 8(5): 471.

4.    Fagiani, E.; Christofori, G. Angiopoietins in angiogenesis. Cancer Lett. 2013, 328, 18–26