96 Tests PN: B111372

Components:

45x Ab-conjugated beads (S5P2 - Human ARTN Ab-bead). PN: B111372A. One vial containing 100 µL of anti-human ARTN conjugated to AimPlex Bead S5P2.

25x Biotin-detection Ab (Human ARTN Biotin-dAb). PN: B111372B. One vial containing    100 µL of biotinylated anti- Human ARTN.

Lyophilized Standard Mix - Human ARTN. PN: B111372S. One vial containing lyophilized ARTN.

Application: Optimal antibody pair and antigen standard for assaying human Human Artemin/ARTN/Neublastin. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 10 pg/mL

  • Quantitation range:

  • LLOQ: < 40 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Sample volume: 15 µL/test

Description:

Artemin (ARTN), also known as Neublastin, is a member of the glial cell-derived neurotrophic factor (GDNF) family of ligands, which also include GDNF, Persephin, and Neurturin. GDNF family ligands are distant members of the TGF-β superfamily. Artemin promotes the survival and growth of various peripheral and central neurons, including sympathetic and dopaminergic neurons. It may also play an important role in the development of sympathetic neurons and several organs. Diseases associated with ARTN include Hirschsprung Disease 1 and Parkinson Disease, Late-Onset

References:

  1. Baloh R.H. et al., Artemin, a novel member of the GDNF ligand family, supports peripheral and central neurons and signals through the GFRalpha3-RET receptor complex. Neuron 1998, PMID: 9883723.

  2. Fernandez R.M.et al., Polymorphisms in the genes encoding the 4 RET ligands, GDNF, NTN, ARTN, PSPN, and susceptibility to Hirschsprung disease. J. Pediatr. Surg. 2008, PMID: 18970938/

  3. Wang X. et al., Structure of artemin complexed with its receptor GFRalpha3: convergent recognition of glial cell line-derived neurotrophic factors. Structure 2006, PMID: 16765900).

  4. Rosenblad C. et al., In vivo protection of nigral dopamine neurons by lentiviral gene transfer of the novel GDNF-family member neublastin/artemin. Mol. Cell. Neurosci. 2000, PMID: 10673327.