96 Tests PN: B113397

Components:

45x Ab-conjugated beads (S5P11 - Human sCD354 Ab-bead). PN: B113397A. One vial containing 100 µL of anti-human sCD354 conjugated to AimPlex Bead S5P11.

25x Biotin-detection Ab (Human sCD354 Biotin-dAb). PN: B113397B. One vial containing 100 µL of biotinylated anti- Human sCD354.

Lyophilized Standard Mix - Human sCD354. PN: B113397S. One vial containing lyophilized sCD354.

Application: Optimal antibody pair and antigen standard for assaying human Human CD354/TREM-1/sCD354. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 20 pg/mL

  • Quantitation range:

  • LLOQ: < 50 pg/mL

  • ULOQ: > 10,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Sample volume: 15 µL/test

Description:

CD354/TREM-1 is highly expressed on peripheral blood myeloid cells (particularly mature monocytes and granulocytes); TREM-1 expression can be further upregulated by bacteria, fungi and lipopolysaccharide. TREM-1 has been shown to interact with the adaptor protein DAP12 to stimulate neutrophil and monocyte-mediated inflammatory responses through the triggering and release of pro-inflammatory cytokines and chemokines such as IL-1β, IL-6, IL-8, MCP-1 and TNF-α. TREM-1 is thought to amplify inflammatory responses to fungal and bacterial infections and a potential biomarker in infectious diseases such as pneumonia and sepsis and inflammatory diseases such as atherosclerosis and rheumatoid arthritis.

References:

  1. Mishra KP, Jain S, Ganju L, Singh SB. Hypoxic Stress Induced TREM-1 and Inflammatory Chemokines in Human Peripheral Blood Mononuclear Cells. Indian J Clin Biochem. 2014 Apr;29(2):133-8. doi: 10.1007/s12291-013-0345-9. Epub 2013 May 28. PMID: 24757292; PMCID: PMC3990790.

  2. Kouassi KT, Gunasekar P, Agrawal DK, Jadhav GP. TREM-1; Is It a Pivotal Target for Cardiovascular Diseases? J Cardiovasc Dev Dis. 2018 Sep 7;5(3):45. doi: 10.3390/jcdd5030045. PMID: 30205488; PMCID: PMC6162371.

  3. Tammaro, A. et al. TREM-1 and its potential ligands in non-infectious diseases: From biology to clinical perspectives. Pharmacol. Ther. 177, 81–95