96 Tests PN: B117789

Components:

45x Ab-conjugated beads (S5P2 - human CLP/Calprotectin(S100A8/A9) Ab-bead). PN: B117789A. One vial containing 100 µL of CLP/Calprotectin(S100A8/A9) conjugated to AimPlex Bead S5P2.

25x Biotin-detection Ab (human CLP/Calprotectin(S100A8/A9) Biotin-dAb). PN: B117789B. One vial containing 100 µL of biotinylated anti-human CLP/Calprotectin(S100A8/A9).

Lyophilized Standard Mix-Human CLP/Calprotectin(S100A8/A9). PN: B117789S. One vial containing lyophilized recombinant CLP/Calprotectin(S100A8/A9).  

Application: Optimal antibody pair and antigen standard for assaying human CLP/Calprotectin(S100A8/A9). To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure. Note: Some specimens may contain high level of CLP/Calprotectin(S100A8/A9). Pre-diluting normal serum and plasma samples 30 folds with the Sample Dilution Buffer (PN: P830100) is recommended for the CLP/Calprotectin(S100A8/A9) assay. Dilution factors should be optimized according to specific experimental conditions and sample types.

Storage: 2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 10 pg/mL

  • Quantitation range:

  • LLOQ: < 20 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Human Group 1: Negligible

  • Sample volume: 15 µL/test

Description:

CLP/Calprotectin(S100A8/A9) is a calcium-binding protein of the S-100 protein family and is found to be induced by activated granulocytes, monocytes, and epithelial cells. CLP has been shown to play an important role in numerous inflammatory diseases. Increasing evidences indicate that CLP is involved in the progression of pulmonary diseases, and its levels may be associated with disease severity and treatment outcomes. It has been found that calprotectin levels positively correlated with neutrophil counts and COVID-19 severities. .

References:

  1. Zeeshan S. The S100 Protein Family as Players and Therapeutic Targets in Pulmonary Diseases. Pulmonary Medicine, Volume 2021, Article ID 5488591.

  2. Wei L, et al. Role of Calprotectin as a Biomarker in Periodontal Disease. Mediators of Inflammation 2019(12):1-10.

  3. Silvin et al. (2020). Elevated calprotectin and abnormal myeloid cell subsets discriminate severe from mild COVID-19. Cell 2020 (182): 1401–1418.