96 Tests PN: B112508

Components:

45x Ab-conjugated beads (S5P9 - Human EG-VEGF Ab-bead). PN: B112508A. One vial containing 100 µL of anti-human EG-VEGF conjugated to AimPlex Bead S5P9.

25x Biotin-detection Ab (Human EG-VEGF Biotin-dAb). PN: B112508B. One vial containing    100 µL of biotinylated anti-Human EG-VEGF.

Lyophilized Standard Mix - Human EG-VEGF. PN: B112508S. One vial containing lyophilized EG-VEGF.

Application: Optimal antibody pair and antigen standard for assaying human Human VEGF/PROK1/PK1. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 15 pg/mL

  • Quantitation range:

  • LLOQ: < 30 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Sample volume: 15 µL/test

Description:

EG-VEGF, a member of the prokineticin family of secreted proteins, is expressed in multiple tissues including the gastrointestinal tract, testis, ovary, placenta and adrenal glands. It binds to the G protein-coupled receptors, EG-VEGF/PK1-R1 and EG-VEGF/PK2-R2, leading to stimulation of smooth muscle contraction and tissue‑specific angiogenesis. The cytokine is expressed in peripheral tissues such as those comprising the circulatory system, lungs, reproductive system, endocrine system and the gastrointestinal system. The protein may be involved in signaling in human fetal ovary during initiation of primordial follicle formation. Sequence variants in this gene may be associated with recurrent miscarriage. Diseases associated with PROK1 include Ectopic Pregnancy and Placental Insufficiency.

References:

  1. Parker R, Liu M, Eyre HJ, et al. (2000). "Y-receptor-like genes GPR72 and GPR73: molecular cloning, genomic organisation and assignment to human chromosome 11q21.1 and 2p14 and mouse chromosome 9 and 6." Biochim. Biophys. Acta. 1491 (1–3): 369–75. doi:10.1016/s0167-4781(00)00023-3. PMID 10760605.

  2. Lin DC, Bullock CM, Ehlert FJ, et al. (2002). "Identification and molecular characterization of two closely related G protein-coupled receptors activated by prokineticins/endocrine gland vascular endothelial growth factor". J. Biol. Chem. 277 (22): 19276–80. doi:10.1074/jbc.M202139200. PMID 11886876.

  3. Masuda Y, Takatsu Y, Terao Y, et al. (2002). "Isolation and identification of EG-VEGF/prokineticins as cognate ligands for two orphan G-protein-coupled receptors". Biochem. Biophys. Res. Commun. 293 (1): 396–402. doi:10.1016/S0006-291X(02)00239-5. PMID 12054613.

  4. Soga T, Matsumoto S, Oda T, et al. (2003). "Molecular cloning and characterization of prokineticin receptors". Biochim. Biophys. Acta. 1579 (2–3): 173–9. doi:10.1016/S0167-4781(02)00546-8. PMID 12427552.