96 Tests PN: B112476

Components:

45x Ab-conjugated beads (S4P7 - Human ENA-78 Ab-bead). PN: B112470A. One vial containing 100 µL of anti-Human ENA-78 conjugated to AimPlex Bead S4P7.

25x Biotin-detection Ab (S4P9 - Human Eotaxin-2 Ab-bead). PN: B112476A. One vial containing 100 µL of anti-Human Eotaxin-2 conjugated to AimPlex Bead S4P9.

Lyophilized Standard Mix-Human Group 6, Panel B, 12-Plex. PN: HG62013. One vial containing lyophilized recombinant Eotaxin-2, IGFBP-1, IGFBP-7, MCP-4, MDC, MIP-1δ, MIP-3α, MIP-3β, IL-6Rα, IL-1RII, Gp130, and TNFRII. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

Application: Optimal antibody pair and antigen standard for assaying human CCL24/SCYA24/MPIF-2/Eotaxin-2. Can be multiplexed with other analytes in Human Group 6.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 10 pg/mL

  • Quantitation range:

  • LLOQ: < 20 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Human Group 6: Negligible

  • Sample volume: 15 µL/test

Description:

Chemokine (C-C motif) ligand 24 (CCL24), also known as Myeloid Progenitor Inhibitory factor 2 (MPIF-2) or eosinophil chemotactic protein 2 (Eotaxin-2) and small inducible cytokine A2 (SYCA2), is a small cytokine belonging to the CC chemokine family. CCL24 interacts with chemokine receptor CCR3 to induce chemotaxis in eosinophils. This chemokine is strongly chemotactic for resting T lymphocytes and slightly chemotactic for neutrophils. Elevated levels of Eotaxin-2 have been seen in patients with aspirin-exacerbated respiratory disease such as asthma. People with lower plasma levels of Eotaxin-2 have not been showing tendency to develop aspirin inducible asthma.

References:

  1. Patel VP, Kreider BL, Li Y, Li H, Leung K, Salcedo T, Nardelli B, Pippalla V, Gentz S, Thotakura R, Parmelee D, Gentz R, Garotta G (April 1997). "Molecular and functional characterization of two novel human C-C chemokines as inhibitors of two distinct classes of myeloid progenitors". J. Exp. Med. 185 (7): 1163–72. doi:10.1084/jem.185.7.1163. PMC 2196270. PMID 9104803.

  2. Hillier LW, Fulton RS, Fulton LA; et al. (July 2003). "The DNA sequence of human chromosome 7". Nature 424 (6945): 157–64. doi:10.1038/nature01782. PMID 12853948.

  3. White JR, Imburgia C, Dul E, Appelbaum E, O'Donnell K, O'Shannessy DJ, Brawner M, Fornwald J, Adamou J, Elshourbagy NA, Kaiser K, Foley JJ, Schmidt DB, Johanson K, Macphee C, Moores K, McNulty D, Scott GF, Schleimer RP, Sarau HM (November 1997). "Cloning and functional characterization of a novel human CC chemokine that binds to the CCR3 receptor and activates human eosinophils". J. Leukoc. Biol. 62 (5): 667–75. PMID 9365122.

  4. Palikhe NS, Kim SH, Cho BY, Ye YM, Choi GS, Park HS (October 2009). "Genetic variability in CRTH2 polymorphism increases eotaxin-2 levels in patients with aspirin exacerbated respiratory disease". Allergy 65 (3): 338–346. doi:10.1111/j.1398-9995.2009.02158.x. PMID 19796209.

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