96 Tests PN: B117728

Components:

45x Ab-conjugated beads (S4P4- Human Ferritin Ab-bead). PN: B117728A. One vial containing 100 µL of anti-human ferritin conjugated to AimPlex Bead S4P4.

25x Biotin-detection Ab (Human Ferritin Biotin-dAb). PN: B117728B. One vial containing 100 µL of biotinylated anti- Human Ferritin.

Lyophilized Standard Mix - Human Ferritin. PN: B117728S. One vial containing lyophilized ferritin.

Application: Optimal antibody pair and antigen standard for assaying human Human Ferritin. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 0.06 ng/mL

  • Quantitation range:

  • LLOQ: < 0.1 ng/mL

  • ULOQ: > 500 ng/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Sample volume: 15 µL/test

Description:

Ferritin is a ubiquitous intracellular protein that stores iron and releases it in a controlled fashion. The protein is produced by almost all living organisms, including algae, bacteria, higher plants, and animals. In humans, it acts as a buffer against iron deficiency and iron overload. Ferritin is found in most tissues as a cytosolic protein, but small amounts are secreted into the serum where it functions as an iron carrier. Plasma ferritin is also an indirect marker of the total amount of iron stored in the body and is used as a diagnostic test for iron deficiency anemia.  Ferritin concentrations increase drastically in the presence of an infection or cancer. Endotoxin is an up-regulator of the gene coding for ferritin, thus causing the concentration of ferritin to rise. By contrast, organisms such as Pseudomonas, although possessing endotoxin, cause serum ferritin levels to drop significantly within the first 48 hours of infection.

References:

  1. Granier T, Langlois d'Estaintot B, Gallois B, Chevalier JM, Précigoux G, Santambrogio P, Arosio P (January 2003). "Structural description of the active sites of mouse L-chain ferritin at 1.2 A resolution". J. Biol. Inorg. Chem. 8 (1–2): 105–11. doi:10.1007/s00775-002-0389-4. PMID 12459904.

  2. PDB: 1r0; Langlois d'Estaintot B, Santambrogio P, Granier T, Gallois B, Chevalier JM, Précigoux G, Levi S, Arosio P (July 2004). "Crystal structure and biochemical properties of the human mitochondrial ferritin and its mutant Ser144Ala". J. Mol. Biol. 340 (2): 277–93. doi:10.1016/j.jmb.2004.04.036. PMID 15201052.

  3. Iron Use and Storage in the Body: Ferritin and Molecular Representations, Rachel Casiday and Regina Frey, Department of Chemistry, Washington University, St. Louis.

  4. Wang W, Knovich MA, Coffman LG, Torti FM, Torti SV (August 2010). "Serum ferritin: Past, present and future". Biochim. Biophys. Acta 1800 (8): 760–9. doi:10.1016/j.bbagen.2010.03.011. PMC 2893236. PMID 20304033.