96 Tests PN: B111380

Components:

45x Ab-conjugated beads (S5P11 - Human Flt-3L Ab-bead). PN: B111380A. One vial containing 100 µL of anti-Human Flt-3L conjugated to AimPlex Bead S5P11.

25x Biotin-detection (Flt-3L Biotin-dAb). PN: B111380B. One vial containing 100 µL of biotinylated anti-Human Flt-3L.

Lyophilized Standard Mix-Human Group 8, Panel B, 11-Plex. PN: HG82011. One vial containing lyophilized recombinant Cystatin C, FGF-1, FGF-19, Flt-3L, GDF-15, Granzyme B, NGAL, PAPP-A, SLPI, RAGE, and ST2.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

Application: Optimal antibody pair and antigen standard for assaying human Human Flt-3 Ligand/Flt-3L/Flk-2L. Can be multiplexed with other analytes in Human Group 8.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 2 pg/mL

  • Quantitation range:

  • LLOQ: < 5 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Human Group 8: Negligible

  • Sample volume: 15 µL/test

Description:

Flt3 (FMS-related tyrosine kinase 3) Ligand (Flt-3) is a growth factor that stimulates the growth and differentiation of early hematopoetic progenitor cells. Flt3 Ligand exerts weak biological effects and synergizes with other colony-stimulating factors to induce the proliferation and differentiation of cells of the myeloid and lymphoid lineages. Studies have suggested that Flt3 Ligand plays an important role in the development and expansion of dendritic cells and that it may influence NK cell survival. Flt-3Ltranscripts have only been found in stromal cells and T cells. It is expressed most often as a trans-membrane cell surface protein, although processing can yield a soluble, secreted isoform. Diseases associated with Flt-3L include vaccinia and aplastic anemia.

References:

  1. Guimond M, Freud AG, Mao HC, Yu J, Blaser BW, Leong JW, Vandeusen JB, Dorrance A, Zhang J, Mackall CL, Caliguri MA. In vivo role of Flt3 ligand and dendritic cells in NK cell homeostasis. J Immunol. 2010 Mar 15; 184(6): 2769-75.

  2. Kingston D, Schmid MA, Onai N, Obata-Onai A, Baumjohann D, Manz MG. The concerted action of GM-CSF and Flt3-ligand on in vivo dendritic cell homeostasis. Blood. 2009 Jul 23; 144(4): 835-43.

  3. Wodnar-Filipowicz A. Flt3 ligand: role in control of hematopoetic and immune functions of the bone marrow. News Physiol Sci. 2003 Dec; 18: 247-51.