96 Tests PN: B113527

Components:

45x Ab-conjugated beads (S5P9 - Human gp130 Ab-bead). PN: B113527A. One vial containing 100 µL of anti-Human gp130 conjugated to AimPlex Bead S5P9.

25x Biotin-detection Ab (Human gp130 Biotin-dAb). PN: B113527B. One vial containing 100 µL of biotinylated anti-Human gp130.

Lyophilized Standard Mix-Human Group 6, Panel B, 12-Plex. PN: HG62013. One vial containing lyophilized recombinant Eotaxin-2, IGFBP-1, IGFBP-7, MCP-4, MDC, MIP-1δ, MIP-3α, MIP-3β, IL-6Rα, IL-1RII, Gp130, and TNFRII. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

Application: Optimal antibody pair and antigen standard for assaying human CCL24/SCYA24/MPIF-2/Eotaxin-2. Can be multiplexed with other analytes in Human Group 6.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 5 pg/mL

  • Quantitation range:

  • LLOQ: < 10 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Human Group 6: Negligible

  • Sample volume: 15 µL/test

Description:

Glycoprotein 130 (also known as Gp130, Interleukin 6 Signal Transducer (IL6ST), IL6-beta or CD130) is a transmembrane protein, the founding member of the class of all cytokine receptors. It forms one subunit of the type I cytokine receptor within the IL-6 receptor family. It is often referred to as the common Gp130 subunit, and is important for signal transduction following cytokine engagement. As with other type I cytokine receptors, Gp130 possesses a WSXWS amino acid motif that ensures correct protein folding and ligand binding. It interacts with Janus kinases to elicit an intracellular signal following receptor interaction with its ligand. Gp130 is an important part of many different types of signaling complexes. Inactivation of Gp130 is lethal to mice. Homozygous mice that are born show a number of defects including impaired development of the ventricular myocardium. Knockout studies in mice suggest that Gp130 plays a critical role in regulating myocyte apoptosis. Hematopoietic effects included reduced numbers of stem cells in the spleen and liver.

References:

  1. Hibi M, Murakami M, Saito M, Hirano T, Taga T, Kishimoto T (1990). "Molecular cloning and expression of an IL-6 signal transducer, gp130". Cell 63 (6): 1149–1157. doi:10.1016/0092-8674(90)90411-7. PMID 2261637.

  2. Bravo J, Staunton D, Heath JK, Jones EY (1998). "Crystal structure of a cytokine-binding region of gp130". EMBO J 17 (6): 1665–1674. doi:10.1093/emboj/17.6.1665. PMC 1170514. PMID 9501088.

  3. Murakami M, Hibi M, Nakagawa N, Nakagawa T, Yasukawa K, Yamanishi K, Taga T, Kishimoto T (1993). "IL-6-induced homodimerization of gp130 and associated activation of a tyrosine kinase". Science 260 (5115): 1808–1810. doi:10.1126/science.8511589. PMID 8511589.

  4. Yoshida K, Taga T, Saito M, Suematsu S, Kumanogoh A, Tanaka T, Fujiwara H, Hirata M, Yamagami T, Nakahata T, Hirabayashi T, Yoneda Y, Tanaka K, Wang WZ, Mori C, Shiota K, Yoshida N, Kishimoto T (1996). "Targeted disruption of gp130, a common signal transducer for the interleukin 6 family of cytokines, leads to myocardial and hematological disorders". Proc. Natl. Acad. Sci. USA 93 (1): 407–411. doi:10.1073/pnas.93.1.407. PMC 40247. PMID 8552649

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