96 Tests PN: B112507

Components:

45x Ab-conjugated beads (S4P7- Human GRO2 ab-bead). PN: B112507A. One vial containing 100 µL of anti-human GRO2 conjugated to AimPlex Bead S4P7.

25x Biotin-detection Ab (Human GRO2 Biotin-dAb). PN B112507B. One vial containing     100 µL of biotinylated anti- Human GRO2.

Lyophilized Standard Mix - Human GRO2. PN: B112507S. One vial containing lyophilized GRO2.

Application: Optimal antibody pair and antigen standard for assaying human Human CXCL2/GROβ/MIP-2α. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 10 pg/mL

  • Quantitation range:

  • LLOQ: < 20 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Sample volume: 15 µL/test

Description:

CXCL2 (Chemokine C-X-C motif ligand 2) is a small cytokine belonging to the CXC chemokine family that is also called macrophage inflammatory protein 2-alpha (MIP2-alpha), Growth-regulated protein beta (Gro-β) and Gro oncogene-2 (Gro-2). CXCL2 is 90% identical in amino acid sequence as a related chemokine, CXCL1. This chemokine is secreted by monocytes and macrophages and is chemotactic for polymorphonuclear leukocytes and hematopoietic stem cells. CXCL2 mobilizes cells by interacting with a cell surface chemokine receptor called CXCR2. Diseases associated with CXCL2 include Keratitis and Acanthamoeba Keratitis.

References:

  1. Wolpe, S. D., Sherry, B., Juers, D., Davatelis, G., Yurt, R. W., Cerami, A. Identification and characterization of macrophage inflammatory protein 2. Proc. Natl. Acad. Sci. 86: 612-616, 1989. PMID 2643119

  2. Iida N., Grotendorst G.R. Cloning and sequencing of a new gro transcript from activated human monocytes: expression in leukocytes and wound tissue. Mol. Cell. Biol. 10:5596-5599, 1990. PMID 2078213

  3. Pelus LM, Fukuda S. Peripheral blood stem cell mobilization: the CXCR2 ligand GRObeta rapidly mobilizes hematopoietic stem cells with enhanced engraftment properties. Exp Hematol. 2006 Aug;34(8):1010-20. PMID 16863907

  4. O'Donovan, N., Galvin, M., Morgan, J. G. Physical mapping of the CXC chemokine locus on human chromosome 4. Cytogenet. Cell Genet. 84: 39-42, 1999. PMID 10343098