96 Tests PN: B112491

Components:

45x Ab-conjugated beads (S4P7 - Human I-309 Ab-bead). PN: B112491A. One vial containing 100 µL of anti-Human I-309 conjugated to AimPlex Bead S4P7.

25x Biotin-detection Ab (Human I-309 Biotin-dAb). PN: B112491B. One vial containing 100 µL of biotinylated anti-Human I-309.

Lyophilized Standard Mix-Human Group 7, Panel A, 8-Plex. PN: HG71008. One vial containing lyophilized recombinant Eotaxin-3, GCP-2, GROα, HCC-1, HCC-4, I-309, MCP-2, and MPIF-1. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

Application: Optimal antibody pair and antigen standard for assaying human CCL1/SCYA1/I-309. Can be multiplexed with other analytes in Human Group 7.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 5 pg/mL

  • Quantitation range:

  • LLOQ: < 10 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Human Group 7: Negligible

  • Sample volume: 15 µL/test

Description:

CCL1 or I-309, also referred to as small inducible cytokine A1 (SYCA1), is a small glycoprotein secreted by activated T cells that belongs to a family inflammatory cytokines known as chemokines.  CCL1 attracts monocytes, NK cells, and immature B cells and dendritic cells by interacting with a cell surface chemokine receptor called CCR8. Diseases associated with CCL1 include HIV-1.

References:

1.      Miller MD, Krangel MS (April 1992). "The human cytokine I-309 is a monocyte chemoattractant". Proc. Natl. Acad. Sci. U.S.A. 89 (7): 2950–4. doi:10.1073/pnas.89.7.2950. PMC 48781. PMID 1557400.

2.      Roos RS, Loetscher M, Legler DF, Clark-Lewis I, Baggiolini M, Moser B (July 1997). "Identification of CCR8, the receptor for the human CC chemokine I-309". J. Biol. Chem. 272 (28): 17251–4. doi:10.1074/jbc.272.28.17251. PMID 9211859.

3.      Sequence and chromosomal location of the I-309 gene. Relationship to genes encoding a family of inflammatory cytokines. (PMID: 2212659) Miller M.D. … Krangel M.S.(J. Immunol. 1990)