96 Tests PN: B114566

Components:

45x Ab-conjugated beads (S5P4 - Human IGFBP-4 Ab-bead). PN: B114566A. One vial containing 100 µL of anti-human IGFBP-4 conjugated to AimPlex Bead S5P4.

25x Biotin-detection Ab (Human IGFBP-4 Biotin-dAb). PN: B114566B. One vial containing 100 µL of biotinylated anti- Human IGFBP-4.

Lyophilized Standard Mix - Human IGFBP-4. PN: B114566S. One vial containing lyophilized IGFBP-4.

Application: Optimal antibody pair and antigen standard for assaying human Human IGFBP-4. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 50 pg/mL

  • Quantitation range:

  • LLOQ: < 100 pg/mL

  • ULOQ: > 50,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Sample volume: 15 µL/test

Description:

Insulin-like growth factor-binding protein 4 (IGFBP-4) binds both insulin-like growth factors (IGFs) I and II. Binding of IGFBPs prolongs the half-life of IGFs and alters their interaction with cell surface receptors. IGFBP-4 inhibits proliferations of several cancer cells in vivo and in vitro. It may act as an apoptotic factor and reduce cancer growth. PAPP-A has been identified as a protease for cleavage of IGFBP-4. A number of studies indicates IGFBP-4 as a marker in autoimmune diseases such as systemic lupus erythematosus and type I diabetes.

References:

  1. Durai R, Yang SY, Seifalian AM, Goldspink G, Winslet MC (2007). "Role of insulin-like growth factor binding protein-4 in prevention of colon cancer". World J Surg Oncol. 5: 128. doi:10.1186/1477-7819-5-128. PMC 2203999. PMID 17988381.

  2. Qin X, Strong DD, Baylink DJ, Mohan S (Sep 1998). "Structure-function analysis of the human insulin-like growth factor binding protein-4". J. Biol. Chem. 273 (36): 23509–16. doi:10.1074/jbc.273.36.23509. PMID 9722589.

  3. Lawrence JB, et al. The insulin-like growth factor (IGF)-dependent IGF binding protein-4 protease secreted by human fibroblasts is pregnancy-associated plasma protein-A. PNAS (1999) 96:3149–53

  4. Wu T, et al. Insulin-like growth factor binding protein-4 as a marker of chronic lupus nephritis. PLoS ONE (2016) 11:e0151491. doi: 10.1371/journal.pone.0151491