96 Tests PN: B113313

Components:

45x Ab-conjugated beads (S5P6- Human IL-13Ralpha1/CD213a1/IL-13RA Ab-bead). PN: B113313A. One vial containing 100 µL of anti-human IL-13Ralpha1/CD213a1/IL-13RA conjugated to AimPlex Bead S5P6.

25x Biotin-detection Ab (human IL-13Ralpha1/CD213a1/IL-13RA Biotin-dAb). PN: B113313B. One vial containing 100 µL of biotinylated anti-Human IL-13Ralpha1/CD213a1/IL-13RA.

Lyophilized Standard - Human IL-13Ralpha1/CD213a1/IL-13RA. PN: B113313S. One vial containing lyophilized IL-13Ralpha1/CD213a1/IL-13RA. 

Application: Optimal antibody pair and antigen standard for assaying human IL-13Ralpha1/CD213a1/IL-13RA. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 100 pg/mL

  • Quantitation range:

  • LLOQ: < 200 pg/mL

  • ULOQ: > 30,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Sample volume: 15 µL/test

Description:

IL-13Ralpha1/CD213a1/IL-13RA is a subunit of the interleukin 13 receptor. IL-13 binds to this subunit with low affinity but forms a high affinity receptor complex with IL4 receptor alpha. This protein has been shown to bind tyrosine kinase TYK2, and thus may mediate the signaling processes that lead to the activation of JAK1, STAT3 and STAT6 induced by IL-13 and IL-4.

References:

  1. Miloux B, et al. Cloning of the human IL-13R alpha1 chain and reconstitution with the IL4R alpha of a functional IL-4/IL-13 receptor complex. FEBS Lett. 401 (2–3): 163–6.

  2. Bieber T, Interleukin-13: Targeting an underestimated cytokine in atopic dermatitis.  Allergy. 2020 Jan;75(1):54-62.