96 Tests PN: B111401

Components:

45x Ab-conjugated beads (S5P8 - human IL-16 Ab-bead). PN: B111401A. One vial containing 100 µL of anti-human IL-16 conjugated to AimPlex Bead S5P8.

25x Biotin-detection Ab (human IL-16 Biotin-dAb). PN: B111401B. One vial containing 100 µL of biotinylated anti-human IL-16

Lyophilized Standard Mix-Human Group 3 Panel B, 12-Plex. PN: HG3012B. One vial containing lyophilized recombinant human IL-9, IL-15, IL-16, IL-17C, IL-23p19, IL-24, IL-27, IL-31, IL-32, IL-34, OSM, and SCF.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

Application: Optimal antibody pair and antigen standard for assaying human IL-16/LCF.  Can be multiplexed with other analytes in Human Group 3.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 5 pg/mL

  • Quantitation range:

  • LLOQ: < 10 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Human Group 3: Negligible

  • Sample volume: 15 µL/test

Description:

Interleukin 16 (Accession Q14005), formerly known as lymphocyte chemoattractant factor, is a pro-inflammatory cytokine produced by both immune and non-immune cells, such as eosinophils, mast cells, keratinocytes, synovial fibroblasts, monocytes. It is even found in the airway epithelial cells of subjects with asthma. During in vitro inflammation observations, IL-16 has been demonstrated to induce cell migration and prepare T cells for proliferation. Rather than utilizing a unique receptor, IL-16 engages a CD4 molecule. It is suggested that IL-16 may be a useful bio-indicator of Systemic lupus erythematosus activity. IL-16 also functions as a suppressor of HIV and SIV infections.

References:

  1. Mak TW, Saunders ME. The Immune Response: Basic and Clinical Principles. 17-Cytokines and Cytokine Receptors. 2006: 463-516. Academic Press.

  2. Cruikshank WW, Kornfeld H, Center DM. Interleukin-16. JLB. 2000; 67(6): 757-766. Doi: 10.1002/jib.67.6.757.

  3. Lee S, Kaneko H, Sekigawa I, Tokano Y, Takasaki Y, Hashimoto H. Circulating interleukin-16 in systemic lupus erythematosus. Rheumatology. 1998; 37(12): 1334-1337. Doi: 10.1093/rheumatology/37.12.1334.