96 Tests PN: A111125

32 Tests PN: A111125T

Components:

45x Ab-conjugated beads (S5P2 - human IL-1α Ab-bead).

  • 96 Tests PN: A111125A. One vial containing 100 µL of anti-human IL-1α conjugated to AimPlex Bead S5P2.

  • 32 Tests PN: A111125TA. One vial containing 35 µL of anti-human IL-1α conjugated to AimPlex Bead S5P2.

25x Biotin-detection Ab (human IL-1α Biotin-dAb).

  • 96 Tests PN: A111125B. One vial containing 100 µL of biotinylated anti-human IL-1α.

  • PN: A111125TB. One vial containing 35 µL of biotinylated anti-human IL-1α.

Lyophilized Standard Mix-Human Group 2 Panel A, 10-Plex.

  • 96 & 32 Tests PN: HG2009A. One vial containing lyophilized recombinant human G-CSF, GM-CSF, IFNα2, IL-1α, IL-3, IL-5, IL-7, IL-11, IL-12p40, and IL-13. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits. 

Application: Optimal antibody pair and antigen standard for assaying human IL-1α.  Can be multiplexed with other analytes in Human Group 2.  To be used in conjunction with the AimPlex NR Basic Kit (96 Tests PN: P100001, 32 Tests) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 2 pg/mL

  • Quantitation range:

  • LLOQ: < 5 pg/mL

  • ULOQ: > 2,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Human Group 2: Negligible

  • Sample volume: 15 µL/test

Description:

Interleukin 1 alpha (Accession P01583), also known as hematopoietin-1 is a member of the Interleukin 1 cytokine family. Originally thought to be just Interleukin-1, IL-1α is in fact a distinct protein from interleukin-1 β. It is a pro-inflammatory cytokine that especially incites fever and sepsis. IL-1α is primarily produced by macrophages, neutrophils, epithelial and endothelial cells. It binds to IL-1 receptor, which also binds to IL-1 receptor antagonist. IL-1α acts as a type of molecular “decision-making nexus” of cells over the severity of damage or infection to their associated tissue. It is also an important cytokine in barrier function of  the epidermis during the aging process.

References:

  1. Barland CO, Zettersten E, Brown BS, Ye J, Elias P, Ghadially R. Imiquimod-Induced Interleukin-1α Stimulating Improves Barrier Homeostasis in Aged Murine Epidermis. J Invest Dermatol. 2004; 122(2): 330-336. Doi: 10.1046/j.0022-202X.2004.22203.x.

  2. Di Paolo NC, Shayakhmetov DM. Interleukin 1α and the inflammatory process. Nat Immunol. 2016; 17(8): 906-913. Doi: 10.1038./ni.3503.

  3. March CJ, et al. Cloning, sequence, and expression of two distinct human interleukin-1 complementary DNAs. Nature. 1985; 315(6021) 641-7. Doi: 10.1038/315641a0.