96 Tests PN: B111161

32 Tests PN: A111161T

Components:

45x Ab-conjugated beads (S5P2 - human IL-22 Ab-bead).

  • 96 Tests PN: B111161A. One vial containing 100 µL of anti-human IL-22 conjugated to AimPlex Bead S5P2.

  • 32 Tests PN: A111161TA. One vial containing 35 µL of anti-human IL-22 conjugated to AimPlex Bead S5P2.

25x Biotin-detection Ab (human IL-22 Biotin-dAb).

  • 96 Tests PN: B111161B. One vial containing 100 µL of biotinylated anti-human IL-22.

  • 32 Tests PN: A111161TB. One vial containing 35 µL of biotinylated anti-human IL-22.

Lyophilized Standard Mix-Human Group 1 Panel B.

  • 96 & 32 Tests PN: HG10011. One vial containing lyophilized recombinant Eotaxin, IL-1β, IL-1RA, IL-8, IL-12p70, IL-22, IP-10, MCP-1, MCP-3, and RANTES. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits. 

Application: Optimal antibody pair and antigen standard for assaying human IL-22.  Can be multiplexed with other analytes in Human Group 1.  To be used in conjunction with the AimPlex NR Basic Kit (96 Tests PN: P100001, 32 Tests PN: P100001T) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 3 pg/mL

  • Quantitation range:

  • LLOQ: <10 pg/mL

  • ULOQ: > 2,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Human Group 1: Negligible

  • Sample volume: 15 µL/test

Description:

Interleukin 22 (Accession Q9GZX6) is a member of the IL10 superfamily, all of which express through cytokine receptor family class II. It is secreted by activated T and NK cells. The receptor that binds to IL-22 is expressed by non-hematopoietic cells, thus IL-22 acts as a signaling protein between lymphocytes and epithelial cells.  It is also an important mediator in tissue repair and epithelial cell proliferation. Diseases associated with IL-22 expression are plaque psoriasis, Behcet’s disease, pneumonia, and asthma.

References:

  1. Honda K. IL-22 from T Cells: Better Late than Never. Immunity. 2012; 37(6): 952-954. Doi:10.1016/j.immuni.2012.11.006.

  2. Tufman A, Huber RM, Volk S, Aigner F, Edelmann M, Gamarra F, Kiefl R, Kahnert K, Tian F, Boulesteix A, Endres S, Kobold S. Interleukin-22 is elevated in lavage from patients with lung cancer and other pulmonary disease. BMC Cancer. 2016; 16(409): 1471-2407. Doi:10.1186./s12885-016-2471-2.

  3. Wook K, Kunz S, Witte E, Friedrich M, Asadullah K, Sabat R. IL-22 Increases the Innate Immunity of Tissues. Immunity. 2004; 21 (2): 241-254. Doi:10.1016/j.immuni.2004.07.007.