96 Tests PN: B111422

Components:

45x Ab-conjugated beads (S5P4 - human IL-34 Ab-bead). PN: B111422A. One vial containing 100 µL of anti-human IL-34 conjugated to AimPlex Bead S5P4.

25x Biotin-detection Ab (human IL-34 Biotin-dAb). PN: B111422B. One vial containing 100 µL of biotinylated anti-human IL-34.

Lyophilized Standard Mix-Human Group 3 Panel B, 12-Plex. PN: HG3012B. One vial containing lyophilized recombinant human IL-9, IL-15, IL-16, IL-17C, IL-23p19, IL-24, IL-27, IL-31, IL-32, IL-34, OSM, and SCF.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

Application: Optimal antibody pair and antigen standard for assaying human IL-34  Can be multiplexed with other analytes in Human Group 3.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 5 pg/mL

  • Quantitation range:

  • LLOQ: < 10 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Human Group 3: Negligible

  • Sample volume: 15 µL/test

Description:

Interleukin 34 (Accession Q6ZMJ4) is a second ligand for CSF-1R. As an interleukin, IL-34 shares some functions with colony-stimulating factor-1. In humans, IL-34 is expressed by mRNA primarily in the spleen and is involved in the proliferation of myeloid cells, macrophage differentiation, and osteoclastogenesis. In mice with IL-34 deficiency, Langerhans cells and microglia were reduced. Overexpression of IL-34 is associated with rheumatoid arthritis, inflammatory bowel disease, and Sjogren’s syndrome.

References:

  1. Hwang S, Choi B, Kang S, et al. Interleukin-34 produced by human fibroblast-like synovial cells in rheumatoid arthritis supports osteoclastogenesis. Arthritis Research & Therapy. 2012; 14(1). Doi: 10.1186/ar3696.

  2. Lin H, Lee E, Hestir K, et al. Discovery of a cytokine and its receptor by functional screening of the extracellular proteome. Science. 2008; 320(5877): 807-811. Doi: 10.1126/science. 1554370. 

  3. Masteller EL, Wong BR. Targeting IL-34 in chronic inflammation. Drug Disc Today. 2014; 19(8): 1212-1216. Doi: 10.1016/j.drudis.2014.05.016.

  4. Zwicker S, Martinez GL, Bosma M, et al. Interleukin 34: a new modulator of human and experimental inflammatory bowel disease. Clin Sci (Lond). 2015; 129(3): 281-290. Doi: 10.1042/CS20150176.