96 Tests PN: A111185

Components:

45x Ab-conjugated beads (S4P9 - human IL-9 Ab-bead). PN: A111185A. One vial containing 100 µL of anti-human IL-9 conjugated to AimPlex Bead S4P9

25x Biotin-detection Ab (human IL-9 Biotin-dAb). PN: A111185B. One vial containing 100 µL of biotinylated anti-human IL-9.

Lyophilized Standard Mix-Human Group 3 Panel B, 12-Plex. PN: HG3012B. One vial containing lyophilized recombinant human IL-9, IL-15, IL-16, IL-17C, IL-23p19, IL-24, IL-27, IL-31, IL-32, IL-34, OSM, and SCF.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

Application: Optimal antibody pair and antigen standard for assaying human IL-9.  Can be multiplexed with other analytes in Human Group 3.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 5 pg/mL

  • Quantitation range:

  • LLOQ: < 10 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Human Group 3: Negligible

  • Sample volume: 15 µL/test

Description:

Interleukin 9 (Accession P15248) is a pleiotropic cytokine and a member of the IL-2 family. Structurally, it is closest related to IL-2 and IL-5. IL-9 is primarily secreted by Th9 cells of CD4+ T cells after being induced by IL-33. It is involved in erythropoiesis and mast cell activation and is possibly involved in erythroid lineage recovery.  IL-9 is strongly associated with airway responsiveness, showing a role as a mediator for regulating physiological responses of asthma.

References:

  1. Humphreys NE, Xu D, Hepworth MR, Liew FY, Grencis RK. IL-33, a potent inducer of adaptive immunity to intestinal nematodes. J Immunol. 2008; 180(4): 2443-9. Doi: 10.4049/jimmunol.180.4.2443.

  2. Louahed J, et al. Interleukin-9 Upregulates Mucus Expression in the Airways. AJRCMB. 2000; 22(6): 3927. Doi: 10.1165/ajrcmb.22.6.3927.

  3. Reche PA. The tertiary structure of γc cytokines dictates receptor sharing. Cytokine. 2019; 116: 161-168. Doi: 10.1016/j.cyto.2019.01.007.

  4. Renauld JC et al. Interleukin-9. Adv Immunol. 1993; 54: 79-97. Doi: 10.1016/S0065-2776(08)60533-7.