96 Tests PN: A112230

Components:

45x Ab-conjugated beads (S5P5 - human I-TAC Ab-bead). PN: A112230A. One vial containing 100 µL of anti-human I-TAC conjugated to AimPlex Bead S5P5

25x Biotin-detection Ab (human I-TAC Biotin-dAb). PN: A112230B. One vial containing 100 µL of biotinylated anti-human I-TAC.

Lyophilized Standard Mix-Human Group 3 Panel A, 8-Plex. PN: HG30008. One vial containing lyophilized recombinant human Fractalkine, LIF, TNFβ, VEGF-D, I-TAC, MIP-1α, MIP-1β, and TIM-1. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

Application: Optimal antibody pair and antigen standard for assaying human I-TAC  Can be multiplexed with other analytes in Human Group 3.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 5 pg/mL

  • Quantitation range:

  • LLOQ: < 10 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Human Group 3: Negligible

  • Sample volume: 15 µL/test

Description:

CXCL11 (Accession O14625) is a CXC family member cytokine. It is expressed primarily in the leukocytes, the pancreas, and liver. It induces chemotaxis in activated T cells and in interaction with CXCR3, CXCL11 inhibit the proliferation of endothelial cells. CXCL11 has been demonstrated to be a biomarker for left ventricular dysfunction and heart failure. Detection of CXCL11 has also helped in differentiating between damaging and benign herpes strains.  Diseases associated with abnormal levels of CXCL11 include autoimmune thyroiditis and endotheliitis.

References:

  1. Thomson AW, Lotze MT. The Cytokine Handbook. 4th Ed. San Diego, California, USA. Academic Press. 2003.

  2. Altara R, Gu Y, Struijker-Boudier HAJ, Thijs L, Staessen JA, Blankesteijn WM. Left Vetnricular Dysfunction and CXCR3 Ligands in Hypertension: From Animal Experiments to a Population-Based Pilot Study. PLoS One. 2015; 10(10): e0141394. Doi: 10.1371/journal.pone.0141394.

  3. Lind L, Studahl M, Persson Berg

  4. Park JW, Z Li, Choi JS, Oh HJ, Park SH, Yoon KC. Expression of CXCL9, -10, and -11 in the aqueous humor of patients with herpetic endotheliitis. Cornea. 2012; 31(11): 1246-50. Doi: 10.1097/ICO.0b013e3182f8eb0.