96 Tests PN: B117490

Components:

45x Ab-conjugated beads (S4P6 - Human Klotho Ab-bead). PN: B117490A. One vial containing 100 µL of anti-human Klotho conjugated to AimPlex Bead S4P6.

25x Biotin-detection Ab (Human Klotho Biotin-dAb). PN: B117490B. One vial containing    100 µL of biotinylated anti- Human Klotho.

Lyophilized Standard Mix - Human Klotho. PN: B117490S. One vial containing lyophilized Klotho.

Application: Optimal antibody pair and antigen standard for assaying human Human Klotho2/KLA/KL. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 20 pg/mL

  • Quantitation range:

  • LLOQ: <40 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Sample volume: 15 µL/test

Description:

Klotho (KLA or KL) is a transmembrane protein that provides some control over the sensitivity of the organism to insulin and appears to be involved in aging. (The name of the gene comes from Klotho or Clotho, one of the Moirai, or Fates, in Greek mythology.) The Klotho protein is a novel β-glucuronidase (EC number 3.2.1.31) capable of hydrolyzing steroid β-glucuronides. Genetic variants in Klotho has been associated with human aging and has been shown to be a circulating factor detectable in serum that declines with age. Klotho-deficient mice can manifest a syndrome resembling accelerated human aging and display extensive and accelerated arteriosclerosis. They also can exhibit impaired endothelium dependent vasodilation and impaired angiogenesis, suggesting that Klotho may protect the cardiovascular system through endothelium-derived NO production.

Although the vast majority of research has been based on the lack of Klotho, it was demonstrated that an overexpression of Klotho in mice might extend their average life span between 19% and 31% compared to normal mice. Variations in the Klotho gene (e.g. SNP Rs9536314) are associated with both life extension and increased cognition in human populations. Diseases associated with KL include Tumoral Calcinosis, Hyperphosphatemic, Familial and Hyperphosphatemic Familial Tumoral Calcinosis (Kl-Related).

References:

  1. Matsumura Y, Aizawa H, Shiraki-Iida T, Nagai R, Kuro-o M, Nabeshima Y (Jan 1998). "Identification of the human klotho gene and its two transcripts encoding membrane and secreted klotho protein". Biochemical and Biophysical Research Communications. 242 (3): 626–30. doi:10.1006/bbrc.1997.8019. PMID 9464267.

  2. Kurosu H, Yamamoto M, Clark JD, Pastor JV, Nandi A, Gurnani P, McGuinness OP, Chikuda H, Yamaguchi M, Kawaguchi H, Shimomura I, Takayama Y, Herz J, Kahn CR, Rosenblatt KP, Kuro-o M (Sep 2005). "Suppression of aging in mice by the hormone Klotho". Science. 309 (5742): 1829–33. Bibcode:2005Sci...309.1829K. doi:10.1126/science.1112766. PMC 2536606. PMID 16123266.

  3. Kuro-o M, Matsumura Y, Aizawa H, Kawaguchi H, Suga T, Utsugi T, Ohyama Y, Kurabayashi M, Kaname T, Kume E, Iwasaki H, Iida A, Shiraki-Iida T, Nishikawa S, Nagai R, Nabeshima YI (Nov 1997). "Mutation of the mouse klotho gene leads to a syndrome resembling ageing". Nature. 390 (6655): 45–51. Bibcode:1997Natur.390...45K. doi:10.1038/36285. PMID 9363890.