96 Tests PN: B115626

Components:

45x Ab-conjugated beads (S4P3 - Human MMP-3 Ab-bead). PN: B115626A. One vial containing 100 µL of anti-human MMP-3 conjugated to AimPlex Bead S4P3.

25x Biotin-detection Ab (Human MMP-3 Biotin-dAb). PN: B115626B. One vial containing 100 µL of biotinylated anti- Human MMP-3.

Lyophilized Standard Mix - Human MMP-3. PN: B115626S. One vial containing lyophilized MMP-3.

Application: Optimal antibody pair and antigen standard for assaying human MMP-3/Stomelysin-1. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 50 pg/mL

  • Quantitation range:

  • LLOQ: <100 pg/mL

  • ULOQ: > 20,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Sample volume: 15 µL/test

Description:

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix and during tissue remodeling in normal physiological processes, such as embryonic development and reproduction, as well as in disease processes, such as arthritis, and tumor metastasis. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation. Nuclear MMP-3 functions as a transcription factor and a proteinase. MMPs play a central role in cell proliferation, migration, differentiation, angiogenesis, apoptosis and host defenses. Dysregulation of MMPs has been implicated in many diseases including arthritis, chronic ulcers, encephalomyelitis and cancer. Tumor metastasis is a multistep process involving the dissemination of tumor cells from the primary tumor to secondarys at a distant organ or tissue. One of the first steps in metastasis is the degradation of the basement membrane, a process in which MMPs have been implicated. MMPs are secreted by tumor cells themselves or by surrounding stromal cells stimulated by the nearby tumor. Numerous studies have linked altered MMP expression in different human cancers with poor disease prognosis. Diseases associated with MMP-3 include coronary heart disease 6 and rheumatoid lung disease.

References:

  1. Ye S, Eriksson P, Hamsten A, Kurkinen M, Humphries SE, Henney AM (May 1996). "Progression of coronary atherosclerosis is associated with a common genetic variant of the human stromelysin-1 promoter which results in reduced gene expression". The Journal of Biological Chemistry 271 (22): 13055–60. doi:10.1074/jbc.271.22.13055. PMID 8662692.

  2. Eguchi T, Kubota S, Kawata K, Mukudai Y, Uehara J, Ohgawara T, Ibaragi S, Sasaki A, Kuboki T, Takigawa M (Apr 2008). "Novel transcription-factor-like function of human matrix metalloproteinase 3 regulating the CTGF/CCN2 gene" (PDF). Molecular and Cellular Biology 28 (7): 2391–2413. doi:10.1128/MCB.01288-07. PMID 18172013.

  3. Matrisian LM (Apr 1990). "Metalloproteinases and their inhibitors in matrix remodeling". Trends in Genetics 6 (4): 121–5. doi:10.1016/0168-9525(90)90126-Q. PMID 2132731.