96 Tests PN: B111192

Components:

45x Ab-conjugated beads (S4P6 - human PDGF-AA Ab-bead). PN: B111192A. One vial containing 100 µL of anti-human PDGF-AA conjugated to AimPlex Bead S4P6.

25x Biotin-detection Ab (human PDGF-AA Biotin-dAb). PN: B111192B. One vial containing 100 µL of biotinylated anti-human PDGF-AA.

Lyophilized Standard Mix-Human Group 10, Panel A, 9-Plex. PN: HG1009A. One vial containing lyophilized recombinant BTC, CNTF, FGF-4, Follistatin, IL-19, KGF, PDGF-AA, PDGF-BB, and PIGF-1.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

Application: Optimal antibody pair and antigen standard for assaying human Human PDGF-AA. Can be multiplexed with other analytes in Human Group 10.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Important: Sample types containing low levels of platelets such as platelet-poor plasma are recommended because PDGF-AA in platelet granules is released upon platelet activation.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, platelet-poor plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 20 pg/mL

  • Quantitation range:

  • LLOQ: < 50 pg/mL

  • ULOQ: > 10,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Human Group 10: Negligible

  • Sample volume: 15 µL/test

Description:

PDGF-AA (platelet-derived growth factor AA) is a member of the PDGF family. The PDGF proteins are derived from four genes (PDGF-A, -B, -C, and -D) that form four disulfide-linked homodimers (PDGF-AA, -BB, -CC, and -DD) and one heterodimer (PDGF-AB). PDGF-AA plays an important role as a mitogen for a number of cell types. Diseases associated with PDGFA include tendinitis and Denys-Drash syndrome.

References:

  1. Stenman G, Rorsman F, Huebner K, Betsholtz C (Sep 1992). "The human platelet-derived growth factor alpha chain (PDGFA) gene maps to chromosome 7p22". Cytogenet Cell Genet 60 (3–4): 206–7. doi:10.1159/000133337. PMID 1505216.

  2. Matsui T, Heidaran M, Miki T, Popescu N, La Rochelle W, Kraus M, Pierce J, Aaronson S (Mar 1989). "Isolation of a novel receptor cDNA establishes the existence of two PDGF receptor genes". Science 243 (4892): 800–4. doi:10.1126/science.2536956. PMID 2536956.

  3. Lue LF, et al. “Converging mediators from immune and trophic pathways to identify Parkinson disease dementia.” Neurol Neuroimmunol Neuroinflamm. 2016 Jan 27;3(1):e193. doi: 10.1212/NXI.0000000000000193. eCollection 2016.