96 Tests PN: B111442

Components:

45x Ab-conjugated beads (S5P11 - human PIGF-1 Ab-bead). PN: B111442A. One vial containing 100 µL of anti-human PIGF-1 conjugated to AimPlex Bead S5P11.

25x Biotin-detection Ab (human PIGF-1 Biotin-dAb). PN: B111442B. One vial containing 100 µL of biotinylated anti-human PIGF-1.

Lyophilized Standard Mix-Human Group 10, Panel A, 9-Plex. PN: HG1009A. One vial containing lyophilized recombinant BTC, CNTF, FGF-4, Follistatin, IL-19, KGF, PDGF-AA, PDGF-BB, and PIGF-1.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

Application: Optimal antibody pair and antigen standard for assaying human Human PIGF/PIG-F. Can be multiplexed with other analytes in Human Group 10.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage: 2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only. Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 5 pg/mL

  • Quantitation range:

  • LLOQ: < 10 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Human Group 10: Negligible

  • Sample volume: 15 µL/test

Description:

PIGF (Phosphatidylinositol-glycan biosynthesis class F protein) is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid which contains three mannose molecules in its core backbone. The GPI-anchor is found on many blood cells and serves to anchor proteins to the cell surface. This protein and another GPI synthesis protein, PIGO, function in the transfer of ethanolaminephosphate (EtNP) to the third mannose in GPI. Diseases associated with PIGF include pre-eclampsia and eclampsia.

References:

  1. Placental growth factor and placental protein 13 in patients with Balkan endemic nephropathy, a worldwide disease, Stefanovic V. et al, Ren Fail.,2015 Aug;37(7):1145-8.

  2. Ohishi K, Inoue N, Endo Y, Fujita T, Takeda J, Kinoshita T (Oct 1995). "Structure and chromosomal localization of the GPI-anchor synthesis gene PIGF and its pseudogene psi PIGF". Genomics 29 (3): 804–7. doi:10.1006/geno.1995.9929. PMID 8575782.

  3. Mechanism of action of antiplatelet drugs on decompression sickness in rats: a protective effect of anti-GPIIbIIIa therapy, Lambrechts K. et al,J Appl Physiol, 2015.

  4. Structure and chromosomal localization of the GPI-anchor synthesis gene PIGF and its pseudogene psi PIGF. Ohishi K et al. Genomics 1995 Oct;29(3):804-807; PMID: 8575782 Europe PMC Pubmed.

  5. Cloning of a human gene, PIG-F, a component of glycosylphosphatidylinositol anchor biosynthesis, by a novel expression cloning strategy. Inoue N et al. J. Biol. Chem. 1993 Apr;268(10):6882-6885; PMID: 8463218 Europe PMC Pubmed.