96 Tests PN: B113365

Components:

45x Ab-conjugated beads (S5P4 - human sSLAMF4 Ab-bead). PN: B113365A. One vial containing 100 µL of anti-human sSLAMF4 conjugated to AimPlex Bead S5P4.

25x Biotin-detection (human sSLAMF4 Biotin-dAb). PN: B113365B. One vial containing 100 µL of biotinylated anti-human sSLAMF4.

Lyophilized Standard Mix-Human Group 9 Panel B, 9-Plex. PN: HG9009B. One vial containing lyophilized recombinant human sCEACAM-1, sALCAM, sSLAMF4, sHVEM, sB7-DC, sB7-H1, sEpCAM, sBLAME and sEPOR. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

Application: Optimal antibody pair and antigen standard for assaying human Human SLAMF4/2B4/NAIL/sCD244. Can be multiplexed with other analytes in Human Group 9.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 20 pg/mL

  • Quantitation range:

  • LLOQ: < 40 pg/mL

  • ULOQ: > 10,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Human Group 9: Negligible

  • Sample volume: 15 µL/test

Description:

SLAMF4, also known as 2B4, NAIL and CD244 is a transmembrane glycoprotein in the SLAM subgroup of the CD2 protein family. SLAM family proteins have an extracellular domain (ECD) with two or four Ig-like domains. SLAMF4 interacts with CD48, while other SLAM family proteins interact homophilically. SLAMF4 is expressed on all NK cells, gamma δ T cells, monocytes, some CD4+ and CD8+ T cells, and some dendritic cells. CD48 mediates SLAMF4+ cell interactions with nearly all hematopoietic cell types, including cells of the same type. SLAMF4/CD48 signaling cooperates with other receptor systems to either promote or inhibit NK and CD8+ T cell activation. The inhibitory activities are distinct from those of MHC I restricted inhibitory NK cell receptors. Ligation of SLAMF4 with antibodies or CD48 constructs can either directly trigger inhibitory signaling or disrupt an inhibitory interaction, leading to cellular activation. Diseases associated with CD244 include rheumatoid arthritis.

References:

  1. Vaidya SV, Mathew PA (2006). "Of mice and men: different functions of the murine and human 2B4 (CD244) receptor on NK cells.". Immunol. Lett. 105 (2): 180–4. doi:10.1016/j.imlet.2006.02.006. PMID 16621032.

  2. Siu G, Strauss EC, Lai E, Hood LE (1986). "Analysis of a human V beta gene subfamily". J. Exp. Med. 164 (5): 1600–14. doi:10.1084/jem.164.5.1600. PMC 2188445. PMID 3772297.

  3. Latchman Y, McKay PF, Reiser H (1998). "Identification of the 2B4 molecule as a counter-receptor for CD48". J. Immunol. 161 (11): 5809–12. PMID 9834056.

  4. Brown MH, Boles K, van der Merwe PA; et al. (1999). "2B4, the Natural Killer and T Cell Immunoglobulin Superfamily Surface Protein, Is a Ligand for CD48". J. Exp. Med. 188 (11): 2083–90. doi:10.1084/jem.188.11.2083. PMC 2212392. PMID 9841922.