96 Tests PN: B113356

Components:

45x Ab-conjugated beads (S5P6 - human Tie-1 -bead). PN: B113356A. One vial containing 100 µL of anti-human Tie-1 conjugated to AimPlex Bead S5P6.

25x Biotin-detection Ab (human Tie-1 Biotin-dAb). PN: B113356B. One vial containing 100 µL of biotinylated anti-human Tie-1.

Lyophilized Standard - Human Tie-1. PN: B113356S. One vial containing lyophilized Tie-1.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

Application: Optimal antibody pair and antigen standard for assaying human Tie-1. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 50 pg/mL

  • Quantitation range:

  • LLOQ: < 100 pg/mL

  • ULOQ: > 50,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Sample volume: 15 µL/test

Description:

Tyrosine kinase with immunoglobulin-like and EGF-like domains 1 also known as Tie-1 is a surface protein expressed on endothelial cells and considered as an orphan receptor. A study found association of elevated serum Tie-1 with cervical carcinoma progression and poor prognosis. Higher levels of serum or plasma Tie-1 were also found in rheumatoid arthritis and metastatic breast cancer.  Tie-1 has a proinflammatory effect and may play a role in the endothelial inflammatory diseases such as atherosclerosis. 

References:

1.    Chen B, et al. Receptor tyrosine kinase Tie-1 overexpression in endothelial cells upregulates adhesion molecules. Biochemical and Biophysical Research Communications. 2008, 371 (3): 475–9.

2.    Bai R. et al. Serum Tie-1 is a Valuable Marker for Predicting the Progression and Prognosis of Cervical Cancer. Pathol Oncol Res. 2021; 27: 1610006

3.    Yang, P, et al. Tie-1: A potential target for anti-angiogenesis therapy. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 35, 615–622 (2015).

4.    Fagiani, E.; Christofori, G. Angiopoietins in angiogenesis. Cancer Lett. 2013, 328, 18–26