96 Tests PN: B113361

Components:

45x Ab-conjugated beads (S5P12 - human Tie-2/Tek/sCD202b-bead). PN: B113361A. One vial containing 100 µL of anti-human Tie-2/Tek/sCD202b conjugated to AimPlex Bead S5P12.

25x Biotin-detection Ab (human Tie-2/Tek/sCD202b Biotin-dAb). PN: B113361B. One vial containing 100 µL of biotinylated anti-human Tie-2/Tek/sCD202b.

Lyophilized Standard - Human Tie-2/Tek/sCD202b. PN: B113361S. One vial containing lyophilized Tie-2/Tek/sCD202b.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

Application: Optimal antibody pair and antigen standard for assaying human Tie-2/Tek/sCD202b. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 50 pg/mL

  • Quantitation range:

  • LLOQ: < 100 pg/mL

  • ULOQ: > 50,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Sample volume: 15 µL/test

Description:

Tie-2/Tek/sCD202b, an angiopoietin receptor, is expressed almost exclusively on vascular endothelial cells. Tie-2 plays an important role in angiogenesis. Serum Tie-2 has been shown to be a potential biomarker for monitoring antiangiogenic therapy. Elevated levels of Tie-2 were found in patients with acute coronary syndrome, gastroenteropancreatic-neuroendocrine tumors with metastases and sepsis.

References:

1.    Fagiani, E.; Christofori, G. Angiopoietins in angiogenesis. Cancer Lett. 2013, 328, 18–26

2.    Partanen J. et al. A novel endothelial cell surface receptor tyrosine kinase with extracellular epidermal growth factor homology domains. Molecular and Cellular Biology. 1992, 12 (4): 1698–707.

3.    Harris et al. Soluble Tie2 and Flt1 Extracellular Domains in Serum of Patients with Renal Cancer and Response to Antiangiogenic Therapy. Clin Cancer Res. 2001, 7: 1992–1997