96 Tests PN: B111461

32 Tests PN: A111461T

Components:

45x Ab-conjugated beads (S5P6 - human TSLP Ab-bead).

  • 96 Tests PN: B111461A. One vial containing 100 µL of anti-human TSLP conjugated to AimPlex Bead S5P6.

  • 32 Tests PN: A111461TA. One vial containing 35 µL of anti-human TSLP conjugated to AimPlex Bead S5P6.

25x Biotin-detection Ab (human TSLP Biotin-dAb).

  • 96 Tests PN: B111461B. One vial containing 100 µL of biotinylated anti-human TSLP.

  • 32 Tests PN: A111461TB. One vial containing 35 µL of biotinylated anti-human TSLP.

Lyophilized Standard Mix-Human Group 2 Panel B, 10-Plex.

  • 96 & 32 Tests PN: HG20010. One vial containing lyophilized recombinant human IL-17F, IL-20, IL-21, IL-28A, IL-29, IL-33, M-CSF, MPO, TSLP, and VEGF-A. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits. 

Application: Optimal antibody pair and antigen standard for assaying human TSLP.  Can be multiplexed with other analytes in Human Group 2.  To be used in conjunction with the AimPlex NR Basic Kit (96 Tests PN: P100001, 32 Tests PN: P100001T) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 2 pg/mL

  • Quantitation range:

  • LLOQ: < 5 pg/mL

  • ULOQ: > 2,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Human Group 2: Negligible

  • Sample volume: 15 µL/test

Description:

Thymic Stromal Lymphopoietin (Accession Q969D9) is a pro-inflammatory member of the IL-2 cytokine family that activates Langerhans cells. TSLP is secreted by hematopoietic cells and some non-hematopoietic cells such as epithelial cells. In both mouse and human studies, TSLP has been associated with the progression of atopic diseases and keratinocytes and stromal cells have been its primary producers during such events. TSLP has also been implicated in the increased likelihood that people with atopic dermatitis will develop allergic rhinitis and asthma later in life. High expression of TSLP has been found in human lung fibroblasts, bronchial epithelial, and smooth muscle cells, indicating a correlation with respiratory disorders.

References:

  1. Soumelis V, Reche PA, Kanzler H, et al. Human epithelial cells trigger dendritic cell mediated allergic inflammation by producing TSLP. Nat Immunol. 2002; 3(7): 673-80. Doi: 10.1038/ni805.

  2. Ziegler SF. The role of thymic stromal lymphopoietin (TSLP) in allergic disorders. Curr Opin Immunol. 2010; 22(6): 795-9. Doi: 10.1016/j.coi.2010.10.020.

  3. Ziegler SF, Roan F, Bell BD, Stoklasek TA, Kitajima