96 Tests PN: B113325

Components:

45x Ab-conjugated beads (S4P3 - human CD23/Fcε RII/sCD23-bead). PN: B113325A. One vial containing 100 µL of anti-human CD23/Fcε RII/sCD23 conjugated to AimPlex Bead S4P3.

25x Biotin-detection Ab (human CD23/Fcε RII/sCD23 Biotin-dAb). PN: B113325B. One vial containing 100 µL of biotinylated anti-human CD23/Fcε RII/sCD23.

Lyophilized Standard - Human CD23/Fcε RII/sCD23. PN: B113325S. One vial containing lyophilized CD23/Fcε RII/sCD23.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

Application: Optimal antibody pair and antigen standard for assaying human CD23/Fcε RII/sCD23. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 100 pg/mL

  • Quantitation range:

  • LLOQ: < 200 pg/mL

  • ULOQ: > 50,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Sample volume: 15 µL/test

Description:

CD23, also known as Fc epsilon RII, or FcεRII (Accession #: P06734), is the "low-affinity" receptor for IgE, an antibody isotype involved in allergy and resistance to parasites, and is important in regulation of IgE levels. It is found on mature B cells, activated macrophages, eosinophils, follicular dendritic cells, and platelets.  Higher levels of sCD23 can be detected in allergic conditions such as asthma, allergic rhinitis and atopic dermatitis and autoimmune diseases such as SLE and rheumatoid arthritis.

References:  

1.    Barna G, et al. The cut-off levels of CD23 expression in the differential diagnosis of MCL and CLL. Hematological Oncology, 2008 26 (3): 167–70. doi:10.1002/hon.855. PMID 18381689. S2CID 20572446.

2.    Henningsson F, et al. IgE-mediated enhancement of CD4+ T cell responses in mice requires antigen presentation by CD11c+ cells and not by B cells. PLOS ONE. 6 (7): e21760. Bibcode:2011PLoSO...621760H. doi:10.1371/journal.pone.0021760. PMC 3130775. PMID 21765910.