96 Tests PN: B113373

Components:

45x Ab-conjugated beads (S5P2 - human sCD265 -bead). PN: B113373A. One vial containing 100 µL of anti-human sCD265 conjugated to AimPlex Bead S5P2.

25x Biotin-detection Ab (human sCD265 Biotin-dAb). PN: B113373B. One vial containing 100 µL of biotinylated anti-human sCD265.

Lyophilized Standard - Human sCD265. PN: B113373S. One vial containing lyophilized soluble CD265.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

Application: Optimal antibody pair and antigen standard for assaying human sCD265. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 50 pg/mL

  • Quantitation range:

  • LLOQ: < 100 pg/mL

  • ULOQ: > 50,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Sample volume: 15 µL/test

Description: CD265/TRANCE-R/RANK/TNFRSF11a/ODFR/sCD265 (Accession Q9Y6Q6) is a member of the tumor necrosis factor receptor family. It is expressed on osteoclast precursors, dendritic cells, and some other immune cells. RANK plays a crucial role in regulating bone metabolism and immune responses. The biological significance of soluble CD265 is still under investigation.

References: 

1.     Anderson DM, et al. A homologue of the TNF receptor and its ligand enhance T-cell growth and dendritic-cell function. Nature. 1997;390(6656):175-179. PMID: 9367155.

2.     Nakagawa N, et al. RANK is the essential signaling receptor for osteoclast differentiation factor in osteoclastogenesis. Biochem Biophys Res Commun. 1998;253(2):395-400. PMID: 9878548.