96 Tests PN: B113332

Components:

45x Ab-conjugated beads (S5P8 - human sCD8alpha-bead). PN: B113332A. One vial containing 100 µL of anti-human sCD8alpha conjugated to AimPlex Bead S5P8.

25x Biotin-detection Ab (human sCD8alpha Biotin-dAb). PN: B113332B. One vial containing 100 µL of biotinylated anti-human sCD8alpha.

Lyophilized Standard - Human CD8alpha. PN: B113332S. One vial containing lyophilized soluble CD8alpha.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

Application: Optimal antibody pair and antigen standard for assaying human CD8alpha. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 20 pg/mL

  • Quantitation range:

  • LLOQ: < 50 pg/mL

  • ULOQ: > 20,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Sample volume: 15 µL/test

Description:

CD8alpha/CD8A/CD8 (Accession P01732) a subunit of the CD8 molecule expressed on cytotoxic T cells. It plays an important role in T cell activation and signaling. Function(s) and biological significance of soluble CD8a are not fully understood and still under investigation.  Studies have found augments of soluble CD8 in HIV diseases and multiple sclerosis

References:  

1.     Parnes JR (1989). Molecular biology and function of CD4 and CD8. Adv. Immunol. Advances in Immunology. 44: 265–311. PMID 2493728

2.     Nishanian P, et al. Serum soluble CD8 molecule is a marker of CD8 T-cell activation in HIV-1 disease. AIDS. 1991. 5(7):805-12PMID: 1909873.

3.     Agostini C, et al. Increased levels of soluble CD8 molecule in the serum of patients with acquired immunodeficiency syndrome (AIDS) and AIDS-related disorders. Clin Immunol Immunopathol. 1989, 50:146-53. PMID: 2491978.

4.     Maimone D, Reder A. Soluble CD8 levels in the CSF and serum of patients with multiple sclerosis. Neurology 1991, 41 (6) 851