96 Tests PN: B111429

Components:

45x Ab-conjugated beads (S5P2 - human EBI3/IL-35 EBI3/IL-27B -bead). PN: B111429A. One vial containing 100 µL of anti-human EBI3/IL-35 EBI3/IL-27B conjugated to AimPlex Bead S5P2.

25x Biotin-detection Ab (human EBI3/IL-35 EBI3/IL-27B Biotin-dAb). PN: B111429B. One vial containing 100 µL of biotinylated anti-human EBI3/IL-35 EBI3/IL-27B.

Lyophilized Standard - Human EBI3/IL-35 EBI3/IL-27B. PN: B111429S. One vial containing lyophilized EBI3/IL-35 EBI3/IL-27B.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

Application: Optimal antibody pair and antigen standard for assaying human EBI3/IL-35 EBI3/IL-27B. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 5 pg/mL

  • Quantitation range:

  • LLOQ: < 10 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Sample volume: 15 µL/test

Description:

Epstein-Barr virus induced gene 3 (EBI3), also known as interleukin-27 subunit beta or IL-27B, is a subunit in 2 distinct heterodimeric cytokines: interleukin-27 and IL-35. It plays a role in regulating cell-mediated immune responses. IL-27 is composed of p28 and EBI3. IL-27 can trigger signaling in T cells, B cells, and myeloid cells. IL-35, an inhibitory cytokine involved in regulatory T-cell function, is composed of EBI3 and the p35 subunit of IL-12.

References:

  1. Collison LW, et al.  The inhibitory cytokine IL-35 contributes to regulatory T-cell function. Nature. 450 (7169): 566–9. doi:10.1038/nature06306. PMID 18033300.

  2. Batten M, et al. Interleukin 27 limits autoimmune encephalomyelitis by suppressing the development of interleukin 17-producing T cells. Nat. Immunol. 7 (9): 929–36. doi:10.1038/ni1375. PMID 16906167.