96 Tests PN: B117333

Components:

45x Ab-conjugated beads (S5P11 - human Granulysin/GNLY/LAG2/TLA519-bead). PN: B117333A. One vial containing 100 µL of anti-human Granulysin/GNLY/LAG2/TLA519 conjugated to AimPlex Bead S5P11.

25x Biotin-detection Ab (human Granulysin/GNLY/LAG2/TLA519 Biotin-dAb). PN: B117333B. One vial containing 100 µL of biotinylated anti-human Granulysin/GNLY/LAG2/TLA519.

Lyophilized Standard - Human Granulysin/GNLY/LAG2/TLA519. PN: B117333S. One vial containing lyophilized Granulysin/GNLY/LAG2/TLA519.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

Application: Optimal antibody pair and antigen standard for assaying human Granulysin/GNLY/LAG2/TLA519. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 20 pg/mL

  • Quantitation range:

  • LLOQ: < 50 pg/mL

  • ULOQ: > 20,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Sample volume: 15 µL/test

Description:

Granulysin/GNLY/LAG2/TLA519 is expressed in most mammals. It is an antimicrobial peptide released by killer lymphocytes in cytotoxic granules.  Granulysin can be a positive or negative influence on the immune response.  In Leprosy, granulysin acts to prevent further infection, and infected individuals often have higher expression of killer cells expressing granulysin.  When granulysin is expressed in high concentrations in autoimmune diseases, individuals can have debilitating or life-threatening symptoms where cells can be lysed by killer cells.  Granulysin has also been shown to slow the progression of cancers and destroy transformed cells through apoptosis.

References:  

1.    Dotiwala F, et al. Granulysin: Killer lymphocyte safeguard against microbes. Current Opinion in Immunology. 60: 19–29. doi:10.1016/j.coi.2019.04.013. PMID  31112765.

2.    Sparrow E, et al. Granulysin: The attractive side of a natural born killer. Immunology Letters. 217: 126–132. doi:10.1016/j.imlet.2019.11.005. PMID 31726187.