96 Tests PN: B117350

Components:

45x Ab-conjugated beads (S4P8 - Human IgG4 Ab-bead). PN: B117350A. One vial containing 100 µL of anti-human IgG4 conjugated to AimPlex Bead S4P8.

25x Biotin-detection Ab Ab (Human IgG4 Biotin-dAb). PN: B117350B. One vial containing 100 µL of biotinylated anti-human IgG4.

Lyophilized Standard Mix - Human IgG4. PN: B117350S. One vial containing lyophilized IgG4.

Application: Optimal antibody pair and antigen standard for assaying human Human IgG4. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Note: Some specimens may contain high level of IgG4. Pre-diluting normal serum and plasma samples 50,000 folds with the Sample Dilution Buffer (PN: P830100) is recommended for the IgG4 assay. Dilution factors should be optimized according to specific experimental conditions and sample types.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 0.2 ng/mL

  • Quantitation range:

  • LLOQ: < 0.5 ng/mL

  • ULOQ: >100 ng/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Sample volume: 15 µL/test