96 Tests PN: C192215
Components:
1x Ab-conjugated beads (premixed).
96 Tests PN: C192215A. One bottle containing 4.7 mL of the following AimPlex antibody-bead conjugates.
S4P2 - anti-H IL-1beta
S4P4 - anti-H IL-6
S4P6 - anti-H IL-1alpha
S4P7 - anti-H IL-2
S4P8 - anti-H IFNgamma
S4P9 - anti-H MIP-1alpha
S4P12 - anti-H IL-12p70
S5P2 - anti-H IL-8
S5P5 - anti-H TNFalpha
S5P6 - anti-H RANTES
S5P7 - anti-H IL-17A
S5P8 - anti-H MCP-1
S5P9 - anti-H IL-18
S5P10 - anti-H IL-10
S5P11 - anti-H IFNalpha2
2x Biotin-detection Ab (premixed).
96 Tests PN: C192215B. One vial containing 1.25 mL of the following biotinylated antibodies.
Biotin - anti-H IL-1beta
Biotin - anti-H IL-6
Biotin - anti-H IL-1alpha
Biotin - anti-H IL-2
Biotin - anti-H IFNgamma
Biotin - anti-H MIP-1alpha
Biotin - anti-H IL-12p70
Biotin - anti-H IL-8
Biotin - anti-H TNFalpha
Biotin - anti-H RANTES
Biotin - anti-H IL-17A
Biotin - anti-H MCP-1
Biotin - anti-H IL-18
Biotin - anti-H IL-10
Biotin - anti-H IFNalpha2
2x NR dAb Diluent.
96 Tests PN: 07NDX21. One vial containing 1.25 mL of biotin detection antibody diluent.
Lyophilized Standard Mix- Human Inflammation 11-Plex.
96 Tests PN: H192215. One vial containing lyophilized recombinant IFNalpha2, IFNgamma, IL-1alpha, IL-1beta, IL-2, IL-6, IL 8, MCP-1, IL-10, IL-12p70, IL-17A, IL-18, MIP-1alpha, RANTES and TNFalpha.
Standard Info Sheet: Indicates reconstitute volume and standard concentrations.
Basic Kit for Premixed Panels:
1x Streptavidin-PE (SAPE).
96 Tests PN: 07SP0011. One bottle containing 2.5 mL of 1x SAPE.
32 Tests PN: 07SP0011T. One bottle containing 1 mL of 1x SAPE.
10x Reading Buffer.
96 Tests PN: 07RB1. One bottle containing 4 mL of 10x buffer.
32 Tests PN: 07RB1T. One bottle containing 1.5 mL of 10x buffer.
10x Wash Buffer.
96 Tests PN: 07WB1. One vial containing 15 mL of 10x buffer.
32 Tests PN: 07WB1T. One vial containing 5 mL of 10x buffer.
PCR 8-tube Strip.
96 & 32 Tests PN: 07P8T1. 2 strips.
Filter Plate and Lid.
96 & 32 Tests PN: 07FPL1. 1 96- well filter plate and 1 plate lid.
Plate Seals.
PN: 07PSR1. 96 Tests: 4 sealers. 32 Tests: 3 sealers.
Application: Optimal antibody pairs and antigen standards for assaying Inflammation 15-Plex Panel. To be used in conjunction with the AimPlex Basic Kit for Premixed Panels - 96 Tests (PN: U100005), and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.
Storage: 2-8 C in the dark.
Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.
Assay Specifications:
Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LODs): IFNalpha2, < 5 pg/mL; IFNgamma, < 3 pg/mL; IL-1alpha, < 2 pg/mL; IL-1beta, < 5 pg/mL; IL-2, < 3 pg/mL; IL-6, < 5 pg/mL; IL-8, < 1 pg/mL; MCP-1, < 2 pg/mL; IL-10, < 2 pg/mL; IL-12p70, < 3 pg/mL; IL-17A, < 3 pg/mL; IL-18, < 5 pg/mL; MIP-1alpha, < 2 pg/mL; RANTES, < 5 pg/mL; TNFalpha, < 1 pg/mL.
LLOQs: IFNalpha2, < 10 pg/mL; IFNgamma, < 10 pg/mL; IL-1alpha, < 5 pg/mL; IL-1beta, < 10 pg/mL; IL-2, < 10 pg/mL; IL-6, < 10 pg/mL; IL-8, < 1 pg/mL; MCP-1, < 5 pg/mL; IL-10, < 5 pg/mL; IL-12p70, < 10 pg/mL; IL-17A, < 10 pg/mL; L-18, < 10 pg/mL; MIP-1alpha, < 5 pg/mL; RANTES, < 10 pg/mL; TNFalpha, < 2 pg/mL.
ULOQs: IFNalpha2, > 5,000 pg/mL; IFNgamma, > 2,000 pg/mL; IL-1alpha, > 2,000 pg/mL; IL-1beta, > 5,000 pg/mL; IL-2, > 2,000 pg/mL; IL-6, > 2,000 pg/mL; IL-8, > 1,000 pg/mL; MCP-1, > 5,000pg/mL; IL-10, > 5,000 pg/mL; IL-12p70, > 2,000 pg/mL; IL-17A, > 1,000 pg/mL; IL-18, > 5,000pg/mL; MIP-1alpha, > 2,000 pg/mL; RANTES, > 5,000 pg/mL; TNFalpha, > 1,000 pg/mL.
Standard dose recovery: 70-130%
Intra-assay CV: < 10%
Inter-assay CV: < 20%
Cross-reactivity of analytes in the Panel: Negligible
Sample volume: 15 µL/test
For Research Use Only. Not for use in diagnostic procedures.