96 Tests PN: B115614

Components:

45x Ab-conjugated beads (S4P10 - human MMP-10-bead). PN: B115614A. One vial containing 100 µL of anti-human MMP-10 conjugated to AimPlex Bead S4P10.

25x Biotin-detection Ab (human MMP-10 Biotin-dAb). PN: B115614B. One vial containing 100 µL of biotinylated anti-human MMP-10.

Lyophilized Standard - Human MMP-10. PN: B115614S. One vial containing lyophilized MMP-10.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

Application: Optimal antibody pair and antigen standard for assaying human MMP-10. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 20 pg/mL

  • Quantitation range:

  • LLOQ: < 100 pg/mL

  • ULOQ: > 20,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Sample volume: 15 µL/test

Description:

Matrix MetalloProteinase-10 (MMP-10) (Accession P09238) is a matrixin, a class of enzymes that belong to the zinc-metalloproteinases family involved in the degradation of the extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. MMP-10 has been linked to cancer stem cell vitality and metastasis. MMP-10 is a potential prognostic biomarker for oral cancer.

References:  

1.    Justilien V, et al. Matrix Metalloproteinase-10 Is Required for Lung Cancer Stem Cell Maintenance, Tumor Initiation and Metastatic Potential. PLOS ONE. 7 (4): e35040.  doi:10.1371/journal.pone.0035040

2.    Upadhyay P, et al. Genomic characterization of tobacco/nut chewing HPV-negative early stage tongue tumors identify MMP10 as a candidate to predict metastases. Oral Oncology. 73: 56–64.