96 Tests PN: B114328

Components:

45x Ab-conjugated beads (S5P7 - Human PCT Ab-bead). PN: B114328A. One vial containing 100 µL of anti-human Activin A conjugated to AimPlex Bead S5P7.

25x Biotin-detection Ab (Human PCT Biotin-dAb). PN: B114328B. One vial containing 100 µL of biotinylated anti-human EGF.

Lyophilized Standard Mix-Human PCT. PN: B114328S. One vial containing lyophilized PCT.

Application: Optimal antibody pair and antigen standard for assaying human Procalcitonin/PCT. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 30 pg/mL

  • Quantitation range:

  • LLOQ: < 50 pg/mL

  • ULOQ: > 50,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Sample volume: 15 µL/test

Description:

Procalcitonin (PCT) is a peptide precursor of the hormone calcitonin, the latter being involved with calcium homeostasis. Measurement of procalcitonin can be used as a marker of severe sepsis caused by bacteria and generally grades well with the degree of sepsis, although levels of procalcitonin in the blood are very low. PCT has the greatest sensitivity (85%) and specificity (91%) for differentiating patients with systemic inflammatory response syndrome (SIRS) from those with sepsis, when compared with IL-2, IL-6, IL-8, CRP and TNF-alpha. Evidence is emerging that procalcitonin levels can reduce unnecessary antibiotic prescribing to people with lower respiratory tract infections. Currently, procalcitonin assays are widely used in the clinical environment

References:

  1. Meisner M, Tschaikowsky K, Palmaers T, Schmidt J (1999). "Comparison of Procalcitonin (PCT) and C-reactive Protein (CRP) Plasma Concentrations at Different SOFA Scores During the Course of Sepsis and MODS". Critical Care. 3 (1): 45–50. PMC 29013 Freely accessible. PMID 11056723. doi:10.1186/cc306.

  2. Balci C, Sungurtekin H, Gürses E, Sungurtekin U, Kaptanoğlu B (February 2003). "Usefulness of Procalcitonin for Diagnosis of Sepsis in the Intensive Care Unit". Critical Care. 7 (1): 85–90. PMC 154110 Freely accessible. PMID 12617745. doi:10.1186/cc1843.

  3. Schuetz P, Christ-Crain M, Thomann R, et al. (September 9, 2009). "Effect of Procalcitonin-based Guidelines vs Standard Guidelines on Antibiotic Use in Lower Respiratory Tract Infections: The ProHOSP Randomized Controlled Trial". JAMA. 302 (10): 1059–1066. PMID 19738090. doi:10.1001/jama.2009.1297.

  4. Yealy DM, Fine MJ (September 9, 2009). "Measurement of Serum Procalcitonin: A Step Closer to Tailored Care for Respiratory Infections?". JAMA. 302 (10): 1115–1116. PMID 19738100. doi:10.1001/jama.2009.1318.