96 Tests PN: B113454

Components:

45x Ab-conjugated beads (S5P3- Human TIM-3/HAVCR2 Ab-bead). PN: B113454A. One vial containing 100 µL of anti-human TIM-3/HAVCR2 conjugated to AimPlex Bead S5P3.

25x Biotin-detection Ab (human TIM-3/HAVCR2 Biotin-dAb). PN: B113454B. One vial containing    100 µL of biotinylated anti-Human TIM-3/HAVCR2.

Lyophilized Standard - Human TIM-3/HAVCR2. PN: B113454S. One vial containing lyophilized TIM-3/HAVCR2. 

Application: Optimal antibody pair and antigen standard for assaying human TIM-3/HAVCR2.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 50 pg/mL

  • Quantitation range:

  • LLOQ: < 100 pg/mL

  • ULOQ: > 20,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Sample volume: 15 µL/test

Description:

T-cell immunoglobulin and mucin-domain containing-3 (TIM-3) (Accession number: Q8TDQ0) is also known as Hepatitis A virus cellular receptor 2 (HAVCR2). HAVCR2 belongs to TIM family cell surface receptor proteins. HAVCR2 is an immune checkpoint and together with other inhibitory receptors including programmed cell death protein 1 (PD-1) and lymphocyte activation gene 3 protein (LAG3) mediate the CD8+ T-cell exhaustion. HAVCR2 expression is up regulated in tumor-infiltrating lymphocytes in lung, gastric, head and neck cancer, schwannoma, melanoma and follicular B-cell non-Hodgkin lymphoma. The role of HAVCR2 in the T-cell dysfunction has been investigated in chronic viral infections.

References:

  1. Jin HT, Anderson AC, Tan WG, West EE, Ha SJ, Araki K, Freeman GJ, Kuchroo VK, Ahmed R. Cooperation of Tim-3 and PD-1 in CD8 T-cell exhaustion during chronic viral infection. Proceedings of the National Academy of Sciences of the United States of America. 2010 Aug. 107 (33): 14733–8. doi:10.1073/pnas.1009731107 

  2. Blackburn SD, Shin H, Haining WN, Zou T, Workman CJ, Polley A, Betts MR, Freeman GJ, Vignali DA, Wherry EJ. Coregulation of CD8+ T cell exhaustion by multiple inhibitory receptors during chronic viral infection. Nature Immunology. 2009 Jan. 10 (1): 29–37. doi:10.1038/ni.1679  

  3. M. Alper Kursunel, Gunes Esendagli. A Co-Inhibitory Alliance in Myeloid Leukemia: TIM-3/Galectin-9 Complex as a New Target for Checkpoint Blockade Therapy. EBioMedicine 2017 Sep; 23: 6–7. Doi: 10.1016/j.ebiom.2017.08.002