96 Tests PN: B111457

Components:

45x Ab-conjugated beads (S5P9- Human TL1A Ab-bead). PN: B111457A. One vial containing 100 µL of anti-human TL1A conjugated to AimPlex Bead S5P9.

25x Biotin-detection Ab (Human TL1A Biotin-dAb). PN: B111457B. One vial containing 100 µL of biotinylated anti- Human TL1A.

Lyophilized Standard Mix - Human TL1A. PN: B111457S. One vial containing lyophilized TL1A.

Application: Optimal antibody pair and antigen standard for assaying human TL1A. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 5 pg/mL

  • Quantitation range:

  • LLOQ: < 10 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Sample volume: 15 µL/test

Description:

TNF-like ligand 1A (TL1A), is also known as TNF superfamily member 15 (TNFSF15), and Vascular endothelial growth inhibitor (VEGI). TL1A is an anti-angiogenic protein. It belongs to tumor necrosis factor (ligand) superfamily. It is expressed in endothelial cells, but not in either B or T cells. Its expression is inducible by TNF-alpha and IL-1 alpha. It is a ligand for receptor TNFRSF25 (death receptor 3) and TNFRSF6B (decoy receptor 3). Through activating NF-κB and MAPK signaling pathways, it acts as an autocrine factor to induce apoptosis in endothelial cells. TNFSF15 polymorphisms has been found to be strongly associated with inflammatory bowel disease. Studies have found that TL1A levels in the serum, synovial fluid and synovial tissues were higher in rheumatoid arthritis and correlated with the severity of rheumatoid arthritis.

References:

  1. Wang EC (Sep 2012). "On death receptor 3 and its ligands…". Immunology. 137 (1): 114–116. doi:10.1111/j.1365-2567.2012.03606.x. PMC 3449252. PMID 22612445.

  2. Zhao Q., Liu T., Hong B., Wang F., Zhou C., Du X., Chen S., Deng X., Duoerkun S., Li Q., et al (2017). “Vascular Endothelial Growth Inhibitor, a Cytokine of the Tumor Necrosis Factor Family, is Associated with Epithelial-Mesenchymal Transition in Renal Cell Carcinoma”. Appl. Immunohistochem. Mol. Morphol. doi: 10.1097/PAI.0000000000000517.

  3. Picornell Y, Mei L, Taylor K, Yang H, Targan SR, Rotter JI (2007). "TNFSF15 is an ethnic-specific IBD gene". Inflammatory Bowel Diseases. 13 (11): 1333–8. doi:10.1002/ibd.20223. PMC 2552994. PMID 17663424.

  4. Kakuta Y, Kinouchi Y, Negoro K, Takahashi S, Shimosegawa T (2006). "Association study of TNFSF15 polymorphisms in Japanese patients with inflammatory bowel disease". Gut. 55 (10): 1527–8. doi:10.1136/gut.2006.100297. PMC 1856432. PMID 16966713.

  5. Cassatella MA, da Silva BP, I. Tinazzi et al., (2007) “Soluble TNF-like cytokine (TL1A) production by immune complexes stimulated monocytes in rheumatoid arthritis,” Journal of Immunology, 178 (11): 7325–7333.

  6. Bamias G, Siakavellas S, Stamatelopoulos K et al. (2008) “Circulating levels of TNF-like cytokine 1A (TL1A) and its decoy receptor 3 (DcR3) in rheumatoid arthritis,” Clinical Immunology, 129 (2): 249–255