96 Tests PN: B111363

Components:

45x Ab-conjugated beads (S4P7 - human YKL40/CHI3L1/Chitinase 3-like 1-bead). PN: B111363A. One vial containing 100 µL of anti-human YKL40/CHI3L1/Chitinase 3-like 1 conjugated to AimPlex Bead S4P7.

25x Biotin-detection Ab (human YKL40/CHI3L1/Chitinase 3-like 1 Biotin-dAb). PN: B111363B. One vial containing 100 µL of biotinylated anti-human YKL40/CHI3L1/Chitinase 3-like 1.

Lyophilized Standard - Human YKL40/CHI3L1/Chitinase 3-like 1. PN: B111363S. One vial containing lyophilized YKL40/CHI3L1/Chitinase 3-like 1.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

Application: Optimal antibody pair and antigen standard for assaying human YKL40/CHI3L1/Chitinase 3-like 1. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 30 pg/mL

  • Quantitation range:

  • LLOQ: < 150 pg/mL

  • ULOQ: > 50,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Sample volume: 15 µL/test

Description:

YKL40, or Chitinase-3-Like Protein 1 (CHI3L1) (Accession #: P36222) is a glycoprotein produced by cancer cells, inflammatory cells and stem cells and plays a role in angiogenesis, cell proliferation and inflammation.  Its levels are elevated in patients with cancer, inflammation, cardiovascular disease, diabetes and infections.

References:  

1.    Blazevic N, et al. YKL-40 as a biomarker in various inflammatory diseases: A review. Biochem Med (Zagreb). 2024 Feb 15; 34(1): 010502.  doi: 10.11613/BM.2024.010502

2.    Tizaoui K, et al. The role of YKL-40 in the pathogenesis of autoimmune diseases: a comprehensive review. Int J Biol Sci. 2022;18:3731–46.