96 Tests PN: B213371

Components:

45x Ab-conjugated beads (S4P7 - Mouse sCD27 Ab-bead). PN: B213371A. One vial containing 100 µL of anti-mouse sCD27 conjugated to AimPlex Bead S4P7.

25x Biotin-detection Ab (Mouse sCD27 Biotin-dAb). PN: B213371B. One vial containing 100 µL of biotinylated anti-mouse sCD27.

Lyophilized Standard Mix - Mouse Group 5 Panel B, 10-Plex. PN: MG5010B. One vial containing lyophilized recombinant sCD27, sCD105, sIFNγR1, sTNFR1, sTNFRII, sTWEAK-R, sVEGFR1, sVEGFR2, sIL-1R1, and sRAGE.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

Application: Optimal antibody pair and antigen standard for assaying human Mouse CD27/sTNFRSF7/sCD27. Can be multiplexed with other analytes in Mouse Group 5. To be used in conjunction with the AimPlex Mouse/Rat Basic Kit (PN: P200201) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage: 2-8 C in the dark

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only. Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 20 pg/mL

  • Quantitation range:

  • LLOQ: < 50 pg/mL

  • ULOQ: > 10,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Mouse Group 5: Negligible

  • Sample volume: 15 µL/test

Description:

CD27, also known as TNFRSF7, is a member of the Tumor Necrosis Factor Receptor SuperFamily. It is currently of interest to immunologists as a co-stimulatory immune checkpoint molecule. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-κB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a pro-apoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. Diseases associated with CD27 include lymphoproliferative syndrome 2 and autosomal recessive lymphoproliferative disease.

References:

  1. Prasad KV, Ao Z, Yoon Y, Wu MX, Rizk M, Jacquot S, Schlossman SF (Jun 1997). "CD27, a member of the tumor necrosis factor receptor family, induces apoptosis and binds to Siva, a proapoptotic protein". Proceedings of the National Academy of Sciences of the United States of America 94 (12): 6346–51. doi:10.1073/pnas.94.12.6346. PMC 21052. PMID 9177220.

  2. Yamamoto H, Kishimoto T, Minamoto S (Nov 1998). "NF-kappaB activation in CD27 signaling: involvement of TNF receptor-associated factors in its signaling and identification of functional region of CD27". Journal of Immunology 161 (9): 4753–9. PMID 9794406.

  3. Akiba H, Nakano H, Nishinaka S, Shindo M, Kobata T, Atsuta M, Morimoto C, Ware CF, Malinin NL, Wallach D, Yagita H, Okumura K (May 1998). "CD27, a member of the tumor necrosis factor receptor superfamily, activates NF-kappaB and stress-activated protein kinase/c-Jun N-terminal kinase via TRAF2, TRAF5, and NF-kappaB-inducing kinase". The Journal of Biological Chemistry 273 (21): 13353–8. doi:10.1074/jbc.273.21.13353. PMID 9582383.