96 Tests PN: B216494

Components:

45x Ab-conjugated beads (S5P7 - Mouse CRP Ab-bead). PN: B216494A. One vial containing 100 µL of anti-mouse CRP conjugated to AimPlex Bead S5P7.

25x Biotin-detection Ab (Mouse CRP Biotin-dAb). PN: B216494B. One vial containing   100 µL of biotinylated anti-mouse CRP.

Lyophilized Standard Mix - Mouse CRP. PN: MCRPS. One vial containing lyophilized CRP.

Application: Optimal antibody pair and antigen standard for assaying human Mouse CRP. To be used in conjunction with the AimPlex Mouse/Rat Basic Kit (PN: P200201) and and the Mouse CRP Diluent kit (PN: P920501). Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Note: Some specimens may contain high level of CRP. For normal serum and plasma samples, diluting samples 4,000 folds with 1x Mouse CRP Assay Buffer is recommended for the CRP assay.  Dilution factors should be optimized according to specific experimental conditions and sample types.

Storage: 2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 30 pg/mL

  • Quantitation range:

  • LLOQ: < 80 pg/mL

  • ULOQ: > 20,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Sample volume: 15 µL/test

Description:

C-reactive protein (CRP) is an annular (ring-shaped), pentameric protein found in blood plasma, whose levels rise in response to inflammation. It is an acute-phase protein of hepatic origin that increases following interleukin-6 secretion by macrophages and T cells. Its physiological role is to bind to lysophosphatidylcholine expressed on the surface of dead or dying cells (and some types of bacteria) in order to activate the complement system via the C1Q complex.  CRP is synthesized by the liver in response to factors released by macrophages and fat cells (adipocytes). It is a member of the pentraxin family of proteins. It is not related to C-peptide (insulin) or protein C (blood coagulation). C-reactive protein was the first pattern recognition receptor (PRR) to be identified. CRP is used mainly as a marker of inflammation. Apart from liver failure, there are few known factors that interfere with CRP production. Measuring and charting CRP values can prove useful in determining disease progress or the effectiveness of treatments. Several studies investigated differential diagnostic values of CRP in a series of inflammatory disease (including inflammatory bowel disease, Intestinal Lymphoma, Intestinal Tuberculosis and Behcet's Syndrome), and compared CRP to other inflammatory biomarkers, such as ESR and WBC.

References:

  1. Thompson D, Pepys MB, Wood SP (Feb 1999). "The physiological structure of human C-reactive protein and its complex with phosphocholine". Structure 7 (2): 169–77. doi:10.1016/S0969-2126(99)80023-9. PMID 10368284.

  2. Pepys MB, Hirschfield GM (Jun 2003). "C-reactive protein: a critical update". The Journal of Clinical Investigation 111 (12): 1805–12. doi:10.1172/JCI18921. PMC 161431. PMID 12813013.

  3. Lau DC, Dhillon B, Yan H, Szmitko PE, Verma S (May 2005). "Adipokines: molecular links between obesity and atheroslcerosis". American Journal of Physiology. Heart and Circulatory Physiology 288 (5): H2031–41. doi:10.1152/ajpheart.01058.2004. PMID 15653761.

  4. Mantovani A, Garlanda C, Doni A, Bottazzi B (Jan 2008). "Pentraxins in innate immunity: from C-reactive protein to the long pentraxin PTX3". Journal of Clinical Immunology 28 (1): 1–13. doi:10.1007/s10875-007-9126-7. PMID 17828584.