96 Tests PN: B211216

Components:

45x Ab-conjugated beads (S4P9 - Mouse FGF-9 Ab-bead).PN: B211216A. One vial containing 100 µL of anti-mouse FGF-9 conjugated to AimPlex Bead S4P9.

25x Biotin-detection Ab (Mouse FGF-9 Biotin-dAb). PN: B211216B. One vial containing 100 µL of biotinylated anti-mouse FGF-9.

Lyophilized Standard Mix - Mouse Group 4 Panel A, 7-Plex. PN: MG4007A. One vial containing lyophilized recombinant FGF-1, FGF-2, FGF-9, HGF, IL-11, IL-20, and IL-33.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

Application: Optimal antibody pair and antigen standard for assaying human Mouse FGF-9/HBGF-9/GAF. Can be multiplexed with other analytes in Mouse Group 4. To be used in conjunction with the AimPlex Mouse/Rat Basic Kit (PN: P200201) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only. Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 10 pg/mL

  • Quantitation range:

  • LLOQ: < 20 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Mouse Group 4: Negligible

  • Sample volume: 15 µL/test

Description:

Fibroblast growth factor 9 (FGF-9, or Glia-activating factor (GAF), possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development. Expression of the mouse homolog of this gene was found to be dependent on Sonic hedgehog signaling. Mice lacking the homolog gene displayed a male-to-female sex reversal phenotype, which suggested a role in testicular embryogenesis. Diseases associated with FGF9 include multiple synostoses syndrome 3 and synovial chondromatosis.

References:

  1. Miyamoto M, Naruo K, Seko C, Matsumoto S, Kondo T, Kurokawa T (Jul 1993). "Molecular cloning of a novel cytokine cDNA encoding the ninth member of the fibroblast growth factor family, which has a unique secretion property". Molecular and Cellular Biology 13 (7): 4251–9. PMC 359975. PMID 8321227.

  2. Santos-Ocampo S, Colvin JS, Chellaiah A, Ornitz DM (Jan 1996). "Expression and biological activity of mouse fibroblast growth factor-9". The Journal of Biological Chemistry 271 (3): 1726–31. doi:10.1074/jbc.271.3.1726. PMID 8576175.

  3. Chellaiah A, Yuan W, Chellaiah M, Ornitz DM (Dec 1999). "Mapping ligand binding domains in chimeric fibroblast growth factor receptor molecules. Multiple regions determine ligand binding specificity". The Journal of Biological Chemistry 274 (49): 34785–94. doi:10.1074/jbc.274.49.34785. PMID 10574949.