96 Tests PN: A211104

32 Tests PN: A211104T

Components:

45x Ab-conjugated beads (S4P7 - mouse GM-CSF Ab-bead). 

  • 96 Tests PN: A211104A. One vial containing 100 µL of anti-mouse GM-CSF conjugated to AimPlex Bead S4P7.

  • 32 Tests PN: A211104TA. One vial containing 35 µL of anti-mouse GM-CSF conjugated to AimPlex Bead S4P7.

25x Biotin-detection Ab (Mouse GM-CSF Biotin-dAb). 

  • 96 Tests PN: A211104B. One vial containing 100 µL of biotinylated anti-mouse GM-CSF.

  • 32 Tests PN: A211104TB. One vial containing 35 µL of biotinylated anti-mouse GM-CSF.

Lyophilized Standard Mix - Group 2 Panel B, 8-Plex. 

  • 96 & 32 Tests PN: MG20008. One vial containing lyophilized recombinant mouse G-CSF, GM-CSF, IL-28A/B, M-CSF, MIP-1α, MIP-1β, OSM, and TSLP.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

Application: Optimal antibody pair and antigen standard for assaying human Mouse GM-CSF/CSF-2. Can be multiplexed with other analytes in Mouse Group 2. To be used in conjunction with the AimPlex Mouse/Rat Basic kit (96 Tests PN:P200201, 32 Tests PN: P200201T) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 5 pg/mL

  • Quantitation range:

  • LLOQ: < 10 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Mouse Group 2: Negligible

  • Sample volume: 15 µL/test

Description:

Granulocyte-Macrophage Colony Stimulating Factor (Accession P01587) is a glycoprotein, acting as a cytokine produced by macrophages, NK cells, mast cells, fibroblasts, and endothelial cells. GM-CSF stimulates the production of granulocytes and monocytes and monocytes. Patients with rheumatoid arthritis have been shown to have high levels in of GM-CSF. GM-CSF has also been shown to play a role in embryonic development by functioning as an embryokine. As female and male embryos react differently to GM-CSF, it may be responsible for alterations in utero. GM-CSF is frequently used in treating leucopenia.

References:

  1. Francisco-Cruz A, Aguilar-Santelises M, et al. Granulocyte-macrophage colony-stimulating factor: not just another haemopoietic growth factor.

  2. Gasson JC. Molecular physiology of granulate-macrophage colony-stimulating factor. Blood. 1991; 77 (6): 1131-1145. PMID: 2001448.

  3. Hansen PJ, Dobbs KB, Denicol AC. Programming of the preimplantation embryo by the embryokine colony stimulating factor 2. Anim Reprod Sci. 2014; 149 (1-2): 59-66. Doi: 10.1016/j.anireprosci.2014.05.017.