96 Tests PN: A211137

Components:

45x Ab-conjugated beads (S4P5 - Mouse IL-17F Ab-bead).  PN: A211137A. One vial containing 100 µL of anti-mouse IL-17F conjugated to AimPlex Bead S4P5.

25x Biotin-detection Ab (Mouse IL-17F Biotin-dAb). PN: A211137B. One vial containing 100 µL of biotinylated anti-mouse IL-17F.

Lyophilized Standard Mix - Mouse Group 2 Panel A, 10-Plex. PN: MG2009A. One vial containing lyophilized recombinant mouse IL-1β, IL-3, IL-5, IL-7, IL-12/IL-23p40, IL-13, IL-15, IL-17F, IL-21, and IL-22.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

Application: Optimal antibody pair and antigen standard for assaying human Mouse IL-17F. Can be multiplexed with other analytes in Mouse Group 2. To be used in conjunction with the AimPlex Mouse/Rat Basic Kit (PN: P200201) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only. Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 5 pgmL

  • Quantitation range:

  • LLOQ: < 10 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Mouse Group 2: Negligible

  • Sample volume: 15 µL/test

Description:

Interleukin 17F (Accession Q7TNI7) is one of six members of the Interleukin-17 family, the others being IL-17A (IL-17), IL-17B, IL-17C, IL-17D, and IL-17E (IL-25). Structurally, IL-17F is most similar to IL-17A and both may compete for the same receptors. IL-17F encourages endothelial cells to produce MCP-1, TGF-beta, and IL-2 and in conjunction with TNFα, induces G-CSF. In vitro has been demonstrated to inhibit angiogenesis. In humans, IL-17F has been associated with asthma, irritable bowel disease, multiple sclerosis, and various lung infections.

References:

  1. Kawaguchi M, Kokubu F, Fujita J, Huang SK, Hizawa N. Role of interleukin-17F in asthma. Inflamm Allergy Drug Targets. 2009; 8(5): 383-9. PMID 20025586.

  2. Chang SH, Dong C. IL-17F: regulation, signaling and function in inflammation: Cytokine. 2009; 46(1): 7-11. Doi: 10.1016.j.cyto.2008.12.024.