96 Tests PN: A211251

Components:

45x Ab-conjugated beads (S5P2 - Mouse IL-7 Ab-bead). PN: B211251A. One vial containing 100 µL of anti-mouse IL-7 conjugated to AimPlex Bead S5P2.

25x Biotin-detection Ab (Mouse IL-7 Biotin-dAb). PN: B211251B. One vial containing 100 µL of biotinylated anti-mouse IL-7.

Lyophilized Standard Mix - Mouse Group 2 Panel A, 10-Plex. PN: MG2009A. One vial containing lyophilized recombinant mouse IL-1β, IL-3, IL-5, IL-7, IL-12/IL-23p40, IL-13, IL-15, IL-17F, IL-21, and IL-22.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

Application: Optimal antibody pair and antigen standard for assaying human Mouse IL-7. Can be multiplexed with other analytes in Mouse Group 2. To be used in conjunction with the AimPlex Mouse/Rat Basic Kit (PN: P200201) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only. Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 2 pgmL

  • Quantitation range:

  • LLOQ: < 5 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Mouse Group 2: Negligible

  • Sample volume: 15 µL/test

Description:

Interleukin 7 (Accession P10168) is a non-hematopoietic growth factor secreted in the thymus and bone marrow by stromal cells. In lymph nodes, IL-7 plays a crucial role in their organogenesis and is essential for homeostatic proliferation of naïve and memory T cells and involved in B cell differentiation. In both mice and humans, deficiency in IL-7 results in low T cell count, indicating immune deficiency. IL-7 promotes hematological malignancies and elevated levels have been found in the plasma of HIV-infected patients. There may be a correlation between IL-7 expression and depression, when blood fraction and gender are taken under consideration. Effects of IL-7 on cancer cells may promote or arrest growth depending upon the cell type.

References:

  1. ElKassar N, Gress RE. An Overview of IL-7 Biology and Its Use in Immunotherapy. J Immunotoxicol. 2010; 7(1): 1-7. Doi:10.3109/15476910903453296.

  2. Hall JR, Weichmann A, Edwards M, Johnson LA, O’Bryant SE. IL-7 and Depression: The importance of gender and blood fraction Behav Brain Res. 2016; 315: 147-9. Doi: 10.1016/j.bbr.2016.08.026.

  3. Gao J, Zhao L, Wan YY, Zhu B. Mechanism of Action of IL-7 and Its Potential Applications and Limitations in Cancer Immunotherapy. Int J Mol Sci. 2015; 16(5): 10267-10280. Doi: 10.3390/ims160510267.

  4. Lin J, Zhu Z, Xiao H, Wakefield MR, Ding VA, Bai Q, Fang Y. The role of Immunity and Cancer. Anticancer Res. 2017; 37(3): 963-967. Doi: 10.21873/anticanres.11405.