96 Tests PN: B211257

Components:

45x Ab-conjugated beads (S4P7 - Mouse LIF Ab-bead). PN: B211257A. One vial containing 100 µL of anti-mouse LIF conjugated to AimPlex Bead S4P7.

25x Biotin-detection Ab (Mouse LIF Biotin-dAb). PN: B211257B. One vial containing 100 µL of biotinylated anti-mouse LIF.

Lyophilized Standard Mix - Mouse Group 3 Panel A, 8-Plex. PN: MG3008A. One vial containing lyophilized recombinant mouse BTC, IL-1RA, IL-9, IL-23p19, IL-25, Leptin, LIF and VEGF.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

Application: Optimal antibody pair and antigen standard for assaying human Mouse Leptin/LEP. Can be multiplexed with other analytes in Mouse Group 3.  To be used in conjunction with the AimPlex Mouse/Rat Basic Kit (PN: P200201) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only. Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 5 pg/mL

  • Quantitation range:

  • LLOQ: < 10 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Mouse Group 3: Negligible

  • Sample volume: 15 µL/test

Description:

Leukemia inhibitory factor (LIF) is an interleukin 6 class cytokine that affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate. LIF derives its name from its ability to induce the terminal differentiation of myeloid leukemic cells, thus preventing their continued growth. Other properties attributed to the cytokine include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation.  It has been suggested that recombinant human LIF might help to improve the implantation rate in women with unexplained infertility.

References:

  1. Wenwei Hu, Zhaohui Feng, Angelika K. Teresky1, Arnold J. Levine (November 29, 2007). "p53 regulates maternal reproduction through LIF". Nature 450 (7170): 721–724. doi:10.1038/nature05993. PMID 18046411.

  2. Aghajanova, L (2004). "Leukemia inhibitory factor and human embryo implantation". Annals of the New York Academy of Sciences 1034 (1): 176–83. doi:10.1196/annals.1335.020. PMID 15731310.

  3. Suman P, Malhotra SS, Gupta SK (Oct 2013). "LIF-STAT signaling and trophoblast biology". JAKSTAT. 2 (4): e25155. doi:10.4161/jkst.25155. PMID 24416645.

  4. Kawahara Y, Manabe T, Matsumoto M, Kajiume T, Matsumoto M, Yuge L (2009). Zwaka, Thomas, ed. "LIF-Free Embryonic Stem Cell Culture in Simulated Microgravity". PLoS ONE 4 (7): e6343. doi:10.1371/journal.pone.0006343. PMC 2710515. PMID 19626124.