96 Tests PN: B212281

Components:

45x Ab-conjugated beads (S5P3 - Mouse MCP-5 A-bead). PN: B212281A. One vial containing 100 µL of anti-mouse MCP-5 conjugated to AimPlex Bead S5P3.

25x Biotin-detection Ab (Mouse MCP-5 Biotin-dAb). PN: B212281B. One vial containing 100 µL of biotinylated anti-mouse MCP-5.

Lyophilized Standard Mix - Mouse Group 3 Panel B, 10-Plex. PN: MG3010B. One vial containing lyophilized recombinant mouse CTACK, CXCL16, LIX, MIP-2, MIP-3α, MCP-5, BAFF, IL-7Rα, RANKL and IL-2Rα.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

Application: Optimal antibody pair and antigen standard for assaying human Mouse CCL12/MCP-5. Can be multiplexed with other analytes in Mouse Group 3. To be used in conjunction with the AimPlex Mouse/Rat Basic Kit (PN: P200201) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only. Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 10 pg/mL

  • Quantitation range:

  • LLOQ: < 20 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Mouse Group 3: Negligible

  • Sample volume: 15 µL/test

Description:

CCL12 (Chemokine C-C motif ligand 12) is a small cytokine belonging to the CC chemokine family that has been described in mice. It is also known as monocyte chemotactic protein 5 (MCP-5) and, due to its similarity with the human chemokine MCP-1, sometimes it is called MCP-1-related chemokine. CCL12 specifically attracts eosinophils, monocytes and lymphocytes. This chemokine is found predominantly in lymph nodes and thymus under normal conditions and its expression can be hugely induced in macrophages. It is thought to coordinate cell movements during early allergic reactions, and immune response to pathogens.

References:

  1. Jia G, Gonzalo J, Lloyd C, Kremer L, Lu L, Martinez-A C, Wershil B, Gutierrez-Ramos J (1996). "Distinct expression and function of the novel mouse chemokine monocyte chemotactic protein-5 in lung allergic inflammation". J Exp Med 184 (5): 1939–51. doi:10.1084/jem.184.5.1939. PMC 2192876. PMID 8920881.

  2. Sarafi M, Garcia-Zepeda E, MacLean J, Charo I, Luster A (1997). "Murine monocyte chemoattractant protein (MCP)-5: a novel CC chemokine that is a structural and functional homologue of human MCP-1". J Exp Med 185 (1): 99–109. doi:10.1084/jem.185.1.99. PMC 2196097. PMID 8996246.