96 Tests PN: B211260

Components:

45x Ab-conjugated beads (S5P9 - Mouse M-CSF Ab-bead). PN: B211260A. One vial containing 100 µL of anti-mouse M-CSF conjugated to AimPlex Bead S5P9.

25x Biotin-detection Ab (Mouse M-CSF Biotin-dAb). PN: B211260B. One vial containing 100 µL of biotinylated anti-mouse M-CSF.

Lyophilized Standard Mix - Mouse Group 2 Panel B, 8-Plex. PN: MG20008. One vial containing lyophilized recombinant mouse G-CSF, GM-CSF, IL-28A/B, M-CSF, MIP-1α, MIP-1β, OSM, and TSLP.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

Application: Optimal antibody pair and antigen standard for assaying human Mouse M-CSF/CSF-1. Can be multiplexed with other analytes in Mouse Group 2. To be used in conjunction with the AimPlex Mouse/Rat Basic Kit (PN: P200201) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 5 pgmL

  • Quantitation range:

  • LLOQ: < 10 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Mouse Group 2: Negligible

  • Sample volume: 15 µL/test

Description:

Macrophage colony-stimulating factor (Accession P07141) is one of three colony-stimulating factors. M-CSF is essential for homeostasis of macrophages, though combined activity with GM-CSF, IL-3, and IFN-γ may increase macrophage growth from marrow cells. M-CSF also exerts paracrine effects on osteoclasts. It has also been demonstrated that high levels of M-CSF are necessary for normal embryonic implementation and placental development. Overall, M-CSF has a role in modulating many hematopoietic progenitors that affect the processes of immunology, metabolism, fertility, and pregnancy. Clinical studies of M-CSF inhibitors against CSFR1 have demonstrated effects on rheumatoid arthritis and neurodegenerative diseases.

References:

  1. Fixe P, Praloran V. Macrophage coloney-stimulating-factor (M-CSF or CSF-1) and its receptor: structure-function relationships. Eur Cytokine Netw. 1997; 8(2): 125-36. PMID: 9262961.

  2. Hume DA, MacDonald KPA. Therapeutic applications of macrophage colony-stimulating factor-1 (CSF-1) and antagonists of CSF-1 receptor (CSF-1R) signaling. Blood. 2012; 119(8): 1810-1820. Doi: 10.1182./blood-2011-09-379214.

  3. Pons V, Rivest S. New Therapeutic Avenues of mCSF for Brain Diseases and Injuries. Front Cell Neurosci. 2018; 12: 499. Doi: 10.3389/fncel.2018.00499.

  4. Stanley ER, Berg KL, Einstein DB, Lee PS, Pixley FJ, Wang Y, Yeung YG. Biology and action of colony-stimulating factor-1. Mol Reprod Dev. 1997; 46(1): 4-10. Doi: 10.1002(SICI)1098.